Basile Lab

Lab and instrumentation

Biodetection and Mass Spectrometry Laboratory

Located in the Physical Sciences Bldg Room 444.

(Mass Spectrometry Instrumentation Lab is located in the Physical Sciences Bldg room 317)

MALDI-ToF/ToF-MS (5800^TM, Sciex) is now available in the Macromolecular Core Facility (MAC) PS104

Our Biodetection and Mass Spectrometry Laboratory has adjacent offices for graduate students.  We are equipped with instrumentation and computers to conduct a wide range of analytical MS measurements and data analyses.  Our laboratory also has an in-house microbiology lab (Biosafety Level 2) where we maintain and produce a wide range of bacterial and viral samples for MS biodetection research.

---

Q-Exactive-HF X a LC-MS/MS high resolution system based on the Orbitrap mass analyzer

Funded by NSF-EPSCoR RII Track-1: Linking microbial life to ecosystem services across Wyoming's dynamic landscape

 

Orbitrap-MS (Q-Exactive HF-X)  with U-HPLC RP (nano, capillary and micro flows)

nanoESI, heated-ESI (HESI) and APCI sources.

GC-High resolution (ToF) MS  (HRT+; Leco); Funded by NSF-EPSCoR RII Track-2 FEC: Insect Cryobiology and Ecophysiology (ICE) Network: integrating genomics, physiology, and modeling

MALDI-ToF/ToF-MS (5800^TM, Sciex) housed  in the Macromolecular Core Facility (MAC) (Funded by NSF-MRI) and LC-fractionation. Used for imaging-MALDI-MS/MS experiment for on-tissue protein identification, polymer characterization and organic synthesis support.

 

MALDI matrix sprayer for imaging MALDI-MS (TM-Sprayer^TM; HTXimaging; NSF-CMI CHE-1611538)

Other supporting instrumentation:

LC-ESI-MSⁿ system: Electrospray Ionization (ESI) Quadrupole ion trap mass spectrometer (LCQ Classic^TM, Finnigan) interfaced with a nano-LC system (Ekspert^TM 425 and NLC400x autosampler).

---

MALDI Time-of-Flight MS

(Voyager DE STR, Applied Biosystems...still operational!)

Kindly donated by Dr. Dale Patterson of Applied Biosystems

 Gas Chromatograph-(single Quad) MS (GC-MS)  

Electron Ionization and Chemical Ionization, single quadrupole MS with liquid injection autosampler (DSQ, Thermo) (Funds:  NSF CAREER award, 2008)

Microwave radiation heating/microwave flow cell

MS Instrumentation Lab in PS 317