Basile Lab
Basile Research Group
Franco Basile, Ph.D., Principal Investigator
Dept 3838
Department of Chemistry
Department of Chemistry
University of Wyoming
1000 E. University Ave.
Office (307) 766-4376
Laramie, WY 82071
Email: basile@uwyo.edu
Located in the Physical Sciences Bldg Room 444.
(Mass Spectrometry Instrumentation Lab is located in the Physical Sciences Bldg room 317)
MALDI-ToF/ToF-MS (5800TM, Sciex) is now available in the Macromolecular Core Facility (MAC) PS104
Our Biodetection and Mass Spectrometry Laboratory has adjacent offices for graduate students. We are equipped with instrumentation and computers to conduct a wide range of analytical MS measurements and data analyses. Our laboratory also has an in-house microbiology lab (Biosafety Level 2) where we maintain and produce a wide range of bacterial and viral samples for MS biodetection research.
Q-Exactive-HF X a LC-MS/MS high resolution system based on the Orbitrap mass analyzer
Funded by NSF-EPSCoR RII Track-1: Linking microbial life to ecosystem services across Wyoming's dynamic landscape
Orbitrap-MS (Q-Exactive HF-X) with U-HPLC RP (nano, capillary and micro flows)
nanoESI, heated-ESI (HESI) and APCI sources.
GC-High resolution (ToF) MS (HRT+; Leco); Funded by NSF-EPSCoR RII Track-2 FEC: Insect Cryobiology and Ecophysiology (ICE) Network: integrating genomics, physiology, and modeling
MALDI-ToF/ToF-MS (5800TM, Sciex) housed in the Macromolecular Core Facility (MAC) (Funded by NSF-MRI) and LC-fractionation. Used for imaging-MALDI-MS/MS experiment for on-tissue protein identification, polymer characterization and organic synthesis support.
MALDI matrix sprayer for imaging MALDI-MS (TM-SprayerTM; HTXimaging; NSF-CMI CHE-1611538)
Other supporting instrumentation:
LC-ESI-MSn system: Electrospray Ionization (ESI) Quadrupole ion trap mass spectrometer (LCQ ClassicTM, Finnigan) interfaced with a nano-LC system (EkspertTM 425 and NLC400x autosampler).
MALDI Time-of-Flight MS
(Voyager DE STR, Applied Biosystems...still operational!)
Kindly donated by Dr. Dale Patterson of Applied Biosystems
Gas Chromatograph-(single Quad) MS (GC-MS)
Electron Ionization and Chemical Ionization, single quadrupole MS with liquid injection autosampler (DSQ, Thermo) (Funds: NSF CAREER award, 2008)
Microwave radiation heating/microwave flow cell
Basile Lab
Basile Research Group
Franco Basile, Ph.D., Principal Investigator
Dept 3838
Department of Chemistry
Department of Chemistry
University of Wyoming
1000 E. University Ave.
Office (307) 766-4376
Laramie, WY 82071
Email: basile@uwyo.edu