Basile Lab
Basile Research Group
Franco Basile, Ph.D., Principal Investigator
Dept 3838
Department of Chemistry
Department of Chemistry
University of Wyoming
1000 E. University Ave.
Office (307) 766-4376
Laramie, WY 82071
Email: basile@uwyo.edu
Research project funded by the National Science Foundation, NSF-IOS: "Why do bacteria make sterols?", PI: N. Ward, co-PI: F. Basile
Metabolomics/Lipidomics of bacteria
This evolutionary biology project aims at elucidating why some bacteria make sterols
Our laboratory implements several MS techniques (GC-MS, GC-MS/MS, LC-ESI-MS/MS and LC offline MALDI-MS/MS) for the analysis of lipids, carbohydrates and metabolites in microorganisms. Recent work from our laboratory identified several hydroxy-fatty acids (some believed to be associated with lipid A) and polyunsaturated fatty acids in the microorganism Gemmata obscuriglobus. (Project in collaboration with Dr. Naomi Ward; Read the article in J. Bacteriology, 2016, 198, 221-236 journal link).
Terbinafine treatment, a suppresor of sterol synthesis, in Gemmata obscuriglobus inhibits normal budding replication (left). Quantitation of sterols in G. obscuriglobus after being treated with terbinafine (right). Figures from: "Essentiality of sterol synthesis genes in the planctomycete bacterium Gemmata obscuriglobus", Elena Rivas-Marin, Sean Stettner, Ekaterina Y. Gottshall, Carlos Santana-Molina, Mitch Helling, Franco Basile, Naomi L. Ward & Damien P. Devos, Nature Communications | (2019) 10:2916 | https://doi.org/10.1038/s41467-019-10983-7
MICROBESTIARY: Understand science thourgh art Microbestiary hopes to show, in welcoming and memorable ways, that the bustling microbial world is populated by strange and beautiful characters that are themselves quite charismatic
Basile Lab
Basile Research Group
Franco Basile, Ph.D., Principal Investigator
Dept 3838
Department of Chemistry
Department of Chemistry
University of Wyoming
1000 E. University Ave.
Office (307) 766-4376
Laramie, WY 82071
Email: basile@uwyo.edu