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Basile Lab

Basile Research Group

Franco Basile, Ph.D., Principal Investigator

Dept 3838

Department of Chemistry

Department of Chemistry

University of Wyoming

1000 E. University Ave.

Office (307) 766-4376

Laramie, WY 82071


Alsike Research Group

Basile Lab resources

Useful Links


Basile pubs word cloud

Book chapters:

(1)        Basile, F. Rapid Sample Preparation for Microorganism Analysis by Mass Spectrometry. In Rapid Characterization of Microorganisms by Mass Spectrometry; Fenselau, C., Demirev, P., Eds.; ACS Symposium series; American Chemical Society: Washington, DC, 2011; Vol. 1065, pp 5–34.

(2)        Basile, F.; Mignon, Rudolph K. Methods and Instrumentation in Mass Spectrometry for the Differentiation of Closely Related Microorganisms. In Applications of Mass Spectrometry in Microbiology:  From Strain Characterization to Rapid Screening for Antibiotic Resistance; Plamen Demirev and Todd Sandrin (Eds); Springer, 2016; pp 13–50.


Peer-reviewed manuscripts:

 (3)        Maus, A.; Bisha, B.; Fagerquist, C. K.; Basile, F. Detection and Identification of a Protein Biomarker in Antibiotic Resistant E. Coli Using Intact Protein LC Offline MALDI-MS and MS/MS. J Appl. Microbiol. 2019, First published: 12 November 2019;

(4)        Rivas-Marin, E.; Stettner, S.; Gottshall, E. Y.; Santana-Molina, C.; Helling, M.; Basile, F.; Ward, N. L.; Devos, D. P. Essentiality of Sterol Synthesis Genes in the Planctomycete Bacterium Gemmata Obscuriglobus. Nat. Commun. 2019, 10 (1), 2916.

(5)        Maus, A.; Mignon, R.; Basile, F. Enhanced Protein Identification Using Graphite-Modified MALDI Plates for Offline LC-MALDI-MS/MS Bottom-up Proteomics. Anal. Biochem. 2018, 545, 31–37.

(6)        Zhou, R.; Basile, F. Plasmonic Thermal Decomposition/Digestion of Proteins: A Rapid On-Surface Protein Digestion Technique for Mass Spectrometry Imaging. Anal. Chem. 2017, 89 (17), 8704–8712.

(7)        Kinde, T. F.; Basile, F.; Dutta, D. Correction to A Microfluidic SPLITT Device for Fractionating Low-Molecular Weight Samples. Anal. Chem. 2017, 89 (3), 2179–2179.

(8)        Kinde, T. F.; Lopez, T. D.; Basile, F.; Dutta, D. Correction to Electrophoretic Extraction of Low Molecular Weight Cationic Analytes from Sodium Dodecyl Sulfate Containing Sample Matrices for Their Direct Electrospray Ionization Mass Spectrometry. Anal. Chem. 2017, 89 (3), 2180–2180.

(9)        Mahat, R. K.; Seebart, C.; Basile, F.; Ward, N. Global and Targeted Lipid Analysis of Gemmata Obscuriglobus Reveals the Presence of Lipopolysaccharide, a Signature of the Classical Gram-Negative Outer Membrane. J Bacteriol. 2016, 198, 221–236.

(10)      Liu, C.; Topchiy, E.; Lehmann, T.; Basile, F. Characterization of the Dehydration Products Due to Thermal Decomposition of Peptides by Liquid Chromatography-Tandem Mass Spectrometry. J. Mass Spectrom. 2015, 50 (3), 625–632.

(11)      Mahat, R. K.; Rodgers, W.; Basile, F. Microwave Radiation Heating in Pressurized Vessels for the Rapid Extraction of Coal Samples for Broad Spectrum GC–MS Analysis. Energy Fuels 2014, 28 (10), 6326–6335.

(12)      Lu, L.; Basile, F. Thermal Decomposition of Phosphorylated Peptides in the Condensed Phase as Studied by Mass Spectrometry. J. Anal. Appl. Pyrolysis 2013, 104, 412–417.

(13)      Toh-Boyo, G. M.; Wulff, S. S.; Basile, F. Comparison of Sample Preparation Methods and Evaluation of Intra- and Intersample Reproducibility in Bacteria MALDI-MS Profiling. Anal. Chem. 2012, 84 (22), 9971–9980.

(14)      Liu, C.; Basile, F. Intermolecular Condensation Products Formed during the Pyrolysis of Peptides. J. Anal. Appl. Pyrolysis 2011, 92 (1), 217–223.

(15)      Basile, F.; Zhang, S.; Kandar, S. K.; Lu, L. Mass Spectrometry Characterization of the Thermal Decomposition/Digestion (TDD) at Cysteine in Peptides and Proteins in the Condensed Phase. J. Am. Soc. Mass Spectrom. 2011, 22 (11), 1926–1940.

(16)      Basile, F.; Sibray, T.; Belisle, J. T.; Bowen, R. A. Analysis of Lipids from Crude Lung Tissue Extracts by Desorption Electrospray Ionization Mass Spectrometry and Pattern Recognition. Anal. Biochem. 2011, 408 (2), 289–296.

(17)      Basile, F.; Hauser, N. Rapid Online Nonenzymatic Protein Digestion Combining Microwave Heating Acid Hydrolysis and Electrochemical Oxidation. Anal. Chem. 2011, 83 (1), 359–367.

(18)      Basile, F.; Zhang, S.; Shin, Y.-S.; Drolet, B. Atmospheric Pressure-Thermal Desorption (AP-TD)/Electrospray Ionization-Mass Spectrometry for the Rapid Analysis of Bacillus Spores. The Analyst 2010, 135 (4), 797.

(19)      Hauser, N. J.; Han, H.; McLuckey, S. A.; Basile, F. Electron Transfer Dissociation of Peptides Generated by Microwave D-Cleavage Digestion of Proteins. J. Proteome Res. 2008, 7 (5), 1867–1872.

(20)      Hauser, N. J.; Basile, F. Online Microwave D-Cleavage LC-ESI-MS/MS of Intact Proteins: Site-Specific Cleavages at Aspartic Acid Residues and Disulfide Bonds. J. Proteome Res. 2008, 7 (3), 1012–1026.

(21)      Zhang, S.; Shin, Y.-S.; Mayer, R.; Basile, F. On-Probe Pyrolysis Desorption Electrospray Ionization (DESI) Mass Spectrometry for the Analysis of Non-Volatile Pyrolysis Products. J. Anal. Appl. Pyrolysis 2007, 80 (2), 353–359.

(22)      Zhang, S.; Basile, F. Site-Specific Pyrolysis-Induced Cleavage at Aspartic Acid Residue in Peptides and Proteins. J. Proteome Res. 2007, 6 (5), 1700–1704.

(23)      Shin, Y.-S.; Drolet, B.; Mayer, R.; Dolence, K.; Basile, F. Desorption Electrospray Ionization-Mass Spectrometry of Proteins. Anal. Chem. 2007, 79 (9), 3514–3518.

(24)      Meetani, M. A.; Shin, Y.-S.; Zhang, S.; Mayer, R.; Basile, F. Desorption Electrospray Ionization Mass Spectrometry of Intact Bacteria. J. Mass Spectrom. 2007, 42 (9), 1186–1193.

(25)      Basile, F.; Kassalainen, G. E.; Ratanathanawongs Williams, S. K. Interface for Direct and Continuous Sample−Matrix Deposition onto a MALDI Probe for Polymer Analysis by Thermal Field Flow Fractionation and Off-Line MALDI-MS. Anal. Chem. 2005, 77 (9), 3008–3012.

(26)      Havey, C. D.; Basile, F.; Mowry, C.; Voorhees, K. J. Evaluation of a Micro-Fabricated Pyrolyzer for the Detection of Bacillus Anthracis Spores. J. Anal. Appl. Pyrolysis 2004, 72 (1), 55–61.

(27)      Fung, K. Y. C.; Askovic, S.; Basile, F.; Duncan, M. W. A Simple and Inexpensive Approach to Interfacing High-Performance Liquid Chromatography and Matrix-Assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry. Proteomics 2004, 4 (10), 3121–3127.

(28)      Meetani, M. A.; Basile, F.; Voorhees, K. J. Investigation of Pyrolysis Residues of Poly(Amino Acids) Using Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry. J. Anal. Appl. Pyrolysis 2003, 68–69, 101–113.

(29)      Basile, F. A Gas Sample Pre‐concentration Device Based on Solid Phase Microextraction (SPME) and Temperature Programmed Desorption (TPD). Instrum. Sci. Technol. 2003, 31 (2), 155–164.

(30)      Basile, F.; Ferrer, I.; Furlong, E. T.; Voorhees, K. J. Simultaneous Multiple Substrate Tag Detection with ESI-Ion Trap MS for In Vivo Bacterial Enzyme Activity Profiling. Anal. Chem. 2002, 74 (16), 4290–4293.

(31)      Madonna, A. J.; Basile, F.; Furlong, E.; Voorhees, K. J. Detection of Bacteria from Biological Mixtures Using Immunomagnetic Separation Combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 2001, 15 (13), 1068–1074.

(32)      Madonna, A. J.; Basile, F.; Ferrer, I.; Meetani, M. A.; Rees, J. C.; Voorhees, K. J. On-Probe Sample Pretreatment for the Detection of Proteins above 15 KDa from Whole Cell Bacteria by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 2000, 14 (23), 2220–2229.

(33)      Basile, F.; Beverly, M. B.; Voorhees, K. J.; Hadfield, T. L. Pathogenic Bacteria: Their Detection and Differentiation by Rapid Lipid Profiling with Pyrolysis Mass Spectrometry. TrAC Trends Anal. Chem. 1998, 17 (2), 95–109.

(34)      Basile, F.; Beverly, M. B.; Abbas-Hawks, C.; Mowry, C. D.; Voorhees, K. J.; Hadfield, T. L. Direct Mass Spectrometric Analysis of in Situ Thermally Hydrolyzed and Methylated Lipids from Whole Bacterial Cells. Anal. Chem. 1998, 70 (8), 1555–1562.

(35)      Basile, F.; Voorhees, K. J.; Hadfield, T. L. Microorganism Gram-Type Differentiation Based on Pyrolysis-Mass Spectrometry of Bacterial Fatty Acid Methyl Ester Extracts. Appl. Environ. Microbiol. 1995, 61 (4), 1534–1539.

(36)      Basile, F.; Hughes, K. D.; Wisniowski, P. E.; Gorenstein, D. G.; Lytle, F. E.; McCay-Buis, T. S.; Huber, D. M.; Hemming, B. C. Fast and Sensitive Laser-Based Enzymatic Detection of the Lactose Operon in Microorganisms. Anal. Biochem. 1993, 211, 55–60.

(37)      Basile, F.; Cardamone, A.; Grinstead, K. D.; Miller, K. J.; Lytle, F. E.; Caprara, A.; Clark, C. D.; Heritier, J.-M. Simple Time-Resolved Fluorometer Based on a Nanosecond Digital Oscilloscope and a Diode-Pumped, Solid-State Laser. Appl. Spectrosc. 1993, 47 (2), 207–210.

(38)      Nowak, S. A.; Basile, F.; Kivi, J. T.; Lytle, F. E. Fast Collection Times for Fluorescence-Decay Measurements. Appl. Spectrosc. 1991, 45 (6), 1026–1030.

(39)      Schwederski, B. E.; Basile-D’Alessandro, F.; Dickson, P. N.; Lee, H. D.; Raycheba, J. M.; Margerum, D. W. Methyl Group Steric Effects on the Kinetics of the Copper (II)-Tripeptide Reactions with Triethylenetetramine. Inorg. Chem. 1989, 28 (18), 3477–3480.


Dissertations from the Basile group: search at UW library

Conference poster presentations:

ASMS 2019 (Atlanta) WP606: Untargeted metabolomics of bumble bee cold tolerance using stacked injection of biphasic extraction with LC-MS/MS, Mitchell Helling ; Kennan J. Oyen ; Michael E. Dillon ; Franco Basile, University of Wyoming, Laramie, WY

ASMS 2018 (San Diego) MP348: Radiative-heating thermal decomposition/digestion for single-step on-tissue digestion of proteins for imaging-MALDI-MS, Authors: Andrew K. Goodenough ; Franco Basile,  University of Wyoming, Laramie, WY

ASMS 2017  (Indianapolis): Characterization of the Lipid A Forms in Gemmata obscuriglobus by MALDI-ToF/ToF-MS, Rudolph Mignon ; Anthony Maus ; Rajendra Mahat ; Corrine Seebart ; Naomi Ward ; Franco Basile*, University of Wyoming, Laramie, WY 

ASMS 2016 (San Antonio):  Differentiation of bacteria at the strain level by MALDI-MS of proteins >15kDa; Franco Basile* ; Bledar Bisha, Anthony Maus, University of Wyoming, Laramie, WY
ASMS 2015 (St. Louis): Liquid Chromatography Direct Electron Ionization Mass Spectrometry for the Analysis of Polycyclic Aromatic Hydrocarbons, Mitchell Helling; Franco Basile; University of Wyoming, Laramie, WY

ASMS 2014 (Baltimore) TP32: Pressurized Heating for the Rapid Preparation/Extraction of Coal Samples for Broad Spectrum GC-MS Analysis, Franco Basile1; Rajendra Mahat1; Wesley Rodgers21University of Wyoming, Laramie, WY; 2JR Simplot Co, Boise, ID

ASMS 2014  (Baltmore) WP01: Photo-Thermal Decomposition/Digestion (Photo-TDD) of Proteins and its Application in MALDI-MS Imaging, Rong Zhou; Franco Basile; University of Wyoming, Laramie, WY

ASMS 2013  (Minneapolis) ThP25: Differentiationof Borrelia species with MALDI-MS using spray sample deposition, Franco Basile1; Gwendoline Toh-Boyo1; Shaun Wulff1; Claudia Molins21University of Wyoming, Laramie, WY; 2Centers for Disease Control and Prevention, Fort Collins, CO

ASMS 2012 (Vancouver) WP09: Evaluation of MALDI Intra Spot and Inter Sample Reproducibility in Bacteria Profiling with MALDI-MS, Franco Basile; Gwendoline Toh-Boyo; Shaun Wulff; University of Wyoming, Laramie, WY



Contact Us

Basile Lab

Basile Research Group

Franco Basile, Ph.D., Principal Investigator

Dept 3838

Department of Chemistry

Department of Chemistry

University of Wyoming

1000 E. University Ave.

Office (307) 766-4376

Laramie, WY 82071


Alsike Research Group

1000 E. University Ave. Laramie, WY 82071
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