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Nuclear Magnetic Resonance Facility|Department of Chemistry

For Solving Simple NMR Problems:

Acquisition Problems

There is one action that any user can safely attempt to resolve acquisition problems
with the NMR.


The ii command: Type ii at the topspin command prompt. If you receive error, warning,
or information messages during the initialization, run ii again.


Locking and Shimming Problems


The rsh command: If you are having problems with locking or shimming, you might try
to read in a standard shim set. This will restore the shim settings to more suitable
values. At the command prompt type rsh. Choose the file BBO with the most recent
date from the list. Choose the “read” button. Lock and shim your sample as usual.

For Setting Up Some Common 1-D NMR Experiments



Experiment: Homo-nuclear decoupling

 

• Acquire a routine 1D proton spectrum.
• Create a new dataset from the 1D proton (new).
• Go to the AcqusPars tab.
Set PULPROG to zghd.2.
• In the “Set Nuclei and Routing” panel (edasp), set NUC2
to 1H and click the default button. Close the panel.
• In the AcqusPars tab:
Set O2 to the frequency of the peak to be decoupled.
Set PLW24 to 0.001 Watt.
• Set the receiver gain (rga).
• Start the experiment (zg).


Solvent pre-saturation

• Acquire a routine 1D proton spectrum.
• Go to the AcqusPars tab.
Set PULPROG to zgpr.
Set D1 to 2.0 sec.
Set O1 to the frequency of the solvent peak.
Set PLW9 = 0.001 Watt. (This may need to be adjusted)
• Set the receiver gain (rga).
• Start the experiment (zg).

Shimming non-deuterated solvents


The command topshim lockoff 1h o1p=x.xx rga
will turn the lock off, set the proton frequency for x.xx
ppm, optimize the receiver gain for the sample and start
gradient auto shimming.


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