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Implementing a Novel Molecular Assay in Uganda for Brucellosis Control

Meagan Soehn

Meagan Soehn, University of Wyoming

Publication Date: 2018
Location: Entebbe, Uganda
Who: Dr. Schumaker’s I.D.E.A (Infectious Disease Epidemiology of Animals) lab  

Research Question
This research will be looking into improving brucellosis control strategies within Uganda. This includes improving surveillance of this disease at a heard level, as well as a preliminary study to identify and characterize the circulating strains of brucellosis in cattle.

Significance
Current disease control measures implemented successfully in developed nations, such as mass vaccination and stamping out procedures, are not feasible in developing nations like Uganda. As eradication takes at least 10 years the plans would severely hurt the community’s economy.

Methods Used
This project aims to fulfill three research objectives. First, we will collect blood samples from a cattle herd with a history of brucellosis infection. These will be tested using RBPT/card tests in the field and ELISA tests later in a laboratory, and the results will determine seroprevalence in this herd. Second, five seropositive animals will have milk and various necropsy samples that will be used on a PCR test to sequence and determine circulating Brucella strains. Finally, a novel diagnostic test, previously developed by our lab, will be evaluated by determining field performance.

Conclusions/Outcomes
Improve both the surveillance and control of brucellosis at the herd level within local Ugandan community.

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