University of Wyoming
Department of Chemistry

Kubelka Group
Protein Folding - Biomolecular Spectroscopy - Computational Chemistry


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Laboratories & Equipment


Protein synthesis & purification

Our model proteins for folding studies are synthesized chemically, using solid-phase FMOC peptide synthesis. Chemical synthesis allows incorporation of 13C isotopically labeled amino acids in specific target positions. It is also straightforward to generate mutated variants of the model proteins. The synthesis is done on a fully automated peptide synthesizer, which allows synthesis of two sequences in parallel and up to 130 unassisted amino acid couplings. Synthetic proteins are purified with by HPLC.


PTI Tribute automated peptide synthesizer


Beckman Gold HPLC


Spectroscopy



PS 119: Spectroscopy lab



PS 119: Spectroscopy lab



Bruker Tensor 27 FTIR



Bruker Vertex 70 step-scanning FTIR


 
Nanodrop UV-VIS spectrophotometer



Sample prep area



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