Streptococcus pneumoniae: Taxos P (optochin) sensitive (zone of inhibition)
Streptococcus mitis: Taxos P resistant (no zone of inhibition)
Streptococcus pyogenes: Taxos A (bacitracin) sensitive (zone of inhibition)
Streptococcus agalactiae: Taxos A resistant (no zone of inhibition)
Escherichia coli
MacConkey: Ferments lactose (striking pink colony).
Glucose tube: Ferments glucose with gas production (yellow with a bubble in the Durham Tube).
SIM: Does not produce H 2S gas, indole positive (rose ring), and may or may not be motile.
KIA: A yellow slant and a yellow butt indicate that both glucose and lactose are fermented. Bubbles, mostly in the butt of the tube, specify gas production.
Proteus mirabilis
MacConkey: Does not ferment lactose (colorless colony).
Glucose tube: Ferments glucose with gas production (yellow with a bubble in Durham Tube).
SIM: Produces H 2S, does not produce indole (no rose ring), and motile (variable with some species/strains).
KIA: A red slant and a yellow butt show that only glucose is fermented. Bubbles and a black precipitate in the butt indicate that gas and H 2S, respectively, are produced.
Shigella dysenteriae
MacConkey: Does not ferment lactose (colorless colony).
Glucose tube: Ferments glucose without producing gas (yellow broth, no bubble in Durham Tube).
SIM: Does not produce H 2S, indole negative, and non-motile.
KIA: A red slant and a yellow butt indicate that only glucose is fermented.
Pseudomonas aeruginosa
MacConkey: Does not ferment lactose (colorless colony).
Glucose tube: Does not ferment glucose (red broth).
SIM: Does not produce H 2S, indole negative, and motile.
A red slant and a red butt show that no fermentation occurs.
Other organisms mentioned
Pseudomonas fluorescens