Lecture #20:
Identification of Bacterial Unknowns III
I. CAMP Test
A. Tests for the ability of an organism to produce the CAMP
factor, a ________________________________________________________.
B. Synergistic test between __________________________________
and _________________________________________.
1. The two bacteria are streaked _____________________________
to one another. They do NOT touch.
2. The CAMP protein ______________________________________
________________________________________________________
of S. aureus by binding to already damaged red blood cells and leading
to complete lysis. As a result, ________________________
of enhanced hemolysis is produced between the two streaks.
See Summary
of Biochemical Tests (CAMP)
The test is ______________________________
S. agalactiae.
II. Methyl Red Voges-Proskauer (MR-VP) Used to determine ____________
_____________________ is used _________________________________
__________________.
A. _________________________________
Fermentation Pathway
1. In this pathway, glucose is fermented to produce several __________________________________________
(lactic, acetic, succinic, and formic acids). The stable production of enough
acid to overcome the phosphate buffer will result in a __________________
_____________________________.
2. ___________________________
is a pH indicator. If this indicator is added to the culture broth and the
pH is below 4.4, a ___________ _______________________________________.
If the MR turns ________________________,
the pH is above 6.0 and the mixed acid fermentation pathway has ____________
been utilized.
See
Summary of Biochemical Tests (MR-VP)
B. ___________________________________
Fermentation Pathway
1. In this pathway, glucose is fermented to produce a _____________
________________________________ instead
of organic acids.
2. In order to detect acetoin, a-napthol
and KOH are added. Acetoin reacts with the a-napthol
in the presence of KOH to produce a red color. Thus, if the culture
is _____________________________,
it will turn “_____________________________________________.”
If the culture is ________________________________,
it will turn “______________________________________________.”
See
Summary of Biochemical Tests (MR-VP)
Note: A culture will usually only be __________________________
_______________________________: either MR+ or VP+. But remember,
some bacteria are nonfermenters and will thus be _______________________________________________.
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