Skip to Main Content

Apply Now to the University of Wyoming apply now
Menu
Contact Us

Dr. Don Jarvis

Department of Molecular Biology

University of Wyoming

Department #3944

1000 E. University Ave.

Laramie, WY 82071

Phone: (307) 766-4282

Email: dljarvis@uwyo.edu

Dr. Don Jarvis

Department of Molecular Biology

Publications

(Last five years)
Mabashi-Asazuma, 
Shi, X., Geisler, C., Kuo, C.-W., Kay-Hooi Khoo, K.-H., and 
Jarvis, D.L. 2013. Impact of a human CMP-sialic acid transporter on recombinant glycoprotein sialylation in glycoengineered insect cells. Glycobiology 23:199-210.
Phillips, A.T., Stauft, C.B., Aboellail, T.A., Toth, A.M., Jarvis, D.L., Powers, A.M., and Olson, K.E. 2013. Bioluminescent imaging and histopathologic characterization of WEEV neuroinvasion in outbred CD-1 mice. PLoS One 8:e53462.
Lin, C.-H. and Jarvis, D.L. 2013. Utility of temporally distinct baculovirus promoters for constitutive and baculovirus-inducible transgene expression in transformed insect cells. J. Biotechnol. 165:11-17.
Serena, M.S., Geisler, C., Metz, G.E., Corva, S.G., Mórtola, E.C., Jarvis, D.L., Echeverría, M.G. 2013. Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs. Prot. Expr. Purif., 90:1-8.
Gao, Y., Aryal, R.P., Ju, T., Cummings, R.D., Gahlay, G., Jarvis, D.L., Matta, K.L., Vlahakis, J.Z., Szarek, W.A., and Brockhausen, I. 2013. Acceptor specificities and selective inhibition of recombinant human Gal- and GlcNAc-transferases that synthesize core structures 1, 2, 3 and 4 of O-glycans. Biochim. Biophys. Acta, 1830:4274-4281.
Gerken, T.A., Revoredo, L., Thome, J., Tabak, L.A., Clausen, H., Vester-Christensen, M.B., 
Jarvis, D.L., Gahlay, G.K., and Moremen, K.W. 2013. The lectin domain of the polypeptide GalNAc transferase family of glycosyltransferases (ppGalNAc T’s) acts as a switch directing glycopeptide substrate glycosylation in an N- or C- direction further controlling mucin-type O-glycosylation. J. Biol. Chem. 288:19900-19914.
An, Y., Rininger, J.A., Jarvis, D.L., Jing, X., Ye, Z., Aumiller, J.J., Eichelberger, M., and Cipollo, J.F. 2013. Comparative glycomics analysis of influenza hemagglutinin (H5N1) produced in vaccine-relevant cell platforms. J. Proteome Res. 12:3707-3720.
Lin, C.-H., Kuo, C.-W., Jarvis, D.L., and Khoo, K.-H. 2013. Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping. Proteomics 14:87-92. PMC3926941.
Phillips, A.T., Schountz, T., Toth, A.M., Rico, A.B., Jarvis, D.L., Powers, A.M., and Olson, K.E. 2013. Liposome-antigen-nucleic acid complexes protect mice from lethal challenge with Western and Eastern equine encephalitis viruses. J. Virol. 88:1771-1780. PMC3911585
Jarvis, D.L. 2014. Recombinant Protein Expression in Baculovirus-Infected Insect Cells. Meth. Enzymol. 536:149-163. PMC in process.
Mabashi-Asazuma, 
H., Kuo, C.-W., Khoo, K.-H. and 
Jarvis, D.L. 2014. A novel baculovirus vector for the production of non-fucosylated recombinant glycoproteins in insect cells. Glycobiology 24:325-340. PMC3919471.
Toth, A.M., Kuo, C.-W., Khoo, K.-H., and Jarvis, D.L. 2014. A new insect cell glycoengineering approach provides baculovirus-inducible glycogene expression and increases human-type glycosylation efficiency. J. Biotechnol. 182-183:19-29. PMC4095979.
Harrison, R.L. and Jarvis, D.L. 2015. Transforming lepidopteran insect cells for continuous recombinant protein expression. Meth. Mol. Biol. 1350:329-348. PMC in process.
Harrison, R.L. and Jarvis, D.L. 2015. Transforming lepidopteran insect cells for improved protein processing and expression. Meth. Mol. Biol. 1350:359-379. PMC in process.
Geisler, C., Mabashi-Asazuma, 
H., Kuo, C.-W., Khoo, K.-H. and Jarvis, D.L. 2015. Engineering ß1,4-galactosyltransferase I to reduce secretion and enhance N-glycan elongation in insect cells. J. Biotechnol. 193:52-65. PMC4278940.
Maghodia, A., Jarvis, D.L., and Geisler, C. 2014. Complete genome sequence of the Autographa californica multiple nucleopolyhedrovirus strain E2. Genome Announc. 2:e01202-01214. PMC4263824.
Geisler, C., Mabashi-Asazuma, H., and Jarvis, D.L. 2015. An overview and history of glycoengineering in insect expression systems. Meth. Mol. Biol. 1321:131-152.
Mabashi-Asazuma, 
H., Sohn, B.-H., Kim, Y.-S., Kuo, C.-W., Khoo, K.-H., Kucharski, C.A., Fraser, M.J., and 
Jarvis, D.L. 2015. Targeted glycoengineering extends the protein N-glycosylation pathway in the silkworm silk gland. Insect Biochem. Mol. Biol. 65:20-27. PMC in process.
Mabashi-Asazuma, 
H., Kuo, C.-W., Khoo, K.-H. and 
Jarvis, D.L. 2015. Modifying an insect cell N-glycan processing pathway using CRISPR-Cas technology. ACS Chem. Biol. 10:2199-2208. PMC in process.
Czuchry, D., Desormeaux, P., Stuart, M., Jarvis, D.L., Matta, K., Szarek, W.A., and Brockhausen, I. 2015. Synthesis of the sialyl-T antigen: Biochemical characterization of a novel α,3-sialyltransferase WbwA from pathogenic Escherichia coli serotype O104 and comparison to human ST3GAL1. J. Bacteriol., 197:3760-3768. PMC4652054.
Revoredo, L., Clausen, H, Moremen, K.W., Jarvis, D.L., Ten Hagen, K.G., Tabak, L.A., and Gerken, T.A. 2015. Mucin type O-glycosylation is controlled and ordered by short and long range glycopeptide substrate recognition that varies among members of the polypeptide GalNAc transferase (ppGalNAc-T) family. Glycobiology 26:360-376. PMC4767052.
Maghodia, A.B., Geisler, C., and Jarvis, D.L. 2016. Characterization of an Sf-rhabdovirus-negative S. frugiperda cell line as an alternative host for recombinant protein production in the baculovirus-insect cell system. Prot. Expr. Purif. 122:45-55. PMC4842140.
Geisler, C. and Jarvis, D.L. 2016. Rhabdovirus-like endogenous viral elements in the genome of Spodoptera frugiperda insect cells are actively transcribed: implications for adventitious virus detection. Biologics 44:219-225. PMC4939140.
Rico, A. Phillips, A.T., Schountz, T, Jarvis, D.L., Tjalkens, R.B., Powers, A.M. and Olson, K.E. 2016. Venezuelan and western equine encephalitis virus E1 liposome antigen nucleic acid complexes protect mice from lethal challenge with multiple alphaviruses. Virology 499:30-39. PMC in process.
Jarvis, D.L., Maghodia, A.B., and Geisler, C. 2016. Assessing and addressing the risks associated with Sf-rhabdovirus, an adventitious agent in the baculovirus-insect cell system. Am. Pharm. Rev. 19:74-77.
Mabashi-Asazuma, H. and Jarvis, D.L. 2017. CRISPR-Cas9 tools for the baculovirus-insect cell system. Proc. Nat’l. Acad. Sci. U.S.A. 114:9068-9073. PMC5576809.
Maghodia, A.B. and Jarvis, D.L. 2017. Characterization of Sf-rhabdovirus variants and their infectivity in different hosts. Virology 512:234-245. PMC5651996.
Geisler, C. and Jarvis, D.L. 2017. Adventitious viruses in insect cell lines used for recombinant protein expression. Prot. Expr. Purif. doi: 10.1016/j.pep.2017.11.002. [Epub ahead of print].
Moremen K.W., Ramiah A., Stuart M., Steel, J., Meng, L., Forouhar, F., Moniz, H.A., Gahlay, G., Gao, Z., Chapla, D., Wang, S., Yang, J.-Y., Prabahkar, P.K., Johnson, R., de la Rosa, M., Geisler, C., Nairn, A.V., Wu, S.-C., Tong, L., Gilbert, H.J., LaBaer, J., and Jarvis, D.L. 2017. Human glycosylation enzymes for enzymatic, structural and functional studies. Nat. Chem. Biol., in press.

(Last update 11/14/17)

Contact Us

Dr. Don Jarvis

Department of Molecular Biology

University of Wyoming

Department #3944

1000 E. University Ave.

Laramie, WY 82071

Phone: (307) 766-4282

Email: dljarvis@uwyo.edu

1000 E. University Ave. Laramie, WY 82071
UW Operators (307) 766-1121 | Contact Us | Download Adobe Reader

Accreditation | Virtual Tour | Emergency Preparedness | Employment at UW | Privacy Policy | Harassment & Discrimination | Accessibility Accessibility information icon