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Department of Molecular Biology|College of Agriculture and Natural Resources

Department of Molecular Biology
University of Wyoming
1000 E. University Ave.
Laramie, WY 82071


Phone: (307) 766-4282

Jarvis Lab: Publications (last five years):

Publications (last five years):
  1. Geisler, C. and Jarvis, D.L. 2010. Identification of genes encoding N-glycan processing β-N-acetylglucosaminidases from Trichoplusia ni and Bombyx mori: implications for N-glycosylation pathway engineering in baculovirus expression systems. Biotechnol. Progr. 26:34-44.
  2. Jarvis, D.L. 2010. Recombinant protein expression in baculovirus-infected insect cells. In Lorsch, J. ed., Methods Navigator Cookbook, Elsevier, Amsterdam.
  3. Hillar, A. and Jarvis, D.L. 2010. Re-visiting the endogenous capacity for recombinant glycoprotein sialylation by baculovirus-infected Tn-4h and DpN1 cells. Glycobiology 20:1323-1330.
  4. Geisler, C. and Jarvis, D.L. 2011. Effective glycoanalysis with Maackia amurensis lectins requires a clear understanding of their binding specificities. Glycobiology 21:988-993.
  5. Toth, A.M., Geisler, C. Aumiller, J.J., and Jarvis, D.L. 2011. Factors affecting recombinant Western equine encephalitis glycoprotein production in the baculovirus system. Protein Expr. Purif. 80:274-282.
  6. Aumiller, J.J., Mabashi-Asazuma, H., Hillar, A., Shi, X., andJarvis, D.L. 2012. A new glycoengineered insect cell line with an inducibly-mammalianized protein N-glycosylation pathway. Glycobiology 22:417-428.
  7. Teulé, F., Miao, Y., Sohn, B.-H., Kim, Y.-S., Hull, J.J., Fraser, M.J., Lewis, R.V. and Jarvis, D.L. 2012. Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties. Proc. Natl. Acad. Sci. U.S.A. 109:923-928.
  8. Geisler, C. and Jarvis, D.L. 2012. Substrate specificities and intracellular distributions of three N-glycan processing enzymes functioning at a key branch point in the insect N-glycosylation pathway. J. Biol. Chem. 287:7084-7097.
  9. Geisler, C., Kotu, V., Sharrow, M., Rendic, D., Poltl, G., Tiemeyer, M., Wilson, I.B.H., and Jarvis, D.L. 2012. The Drosophila neurally altered carbohydrate mutant has a defective Golgi GDP-fucose transporter. J. Biol. Chem. 287:29599-29609.
  10. Geisler, C. and Jarvis, D.L. 2012. Innovative use of a bacterial enzyme involved in sialic acid degradation to initiate sialic acid biosynthesis in glycoengineered insect cells. Metabol. Engr. 14:642-652.
  11. Mabashi-Asazuma, Shi, X., Geisler, C., Kuo, C.-W., Kay-Hooi Khoo, K.-H., and Jarvis, D.L. 2013. Impact of a human CMP-sialic acid transporter on recombinant glycoprotein sialylation in glycoengineered insect cells. Glycobiology 23:199-210.
  12. Phillips, A.T., Stauft, C.B., Aboellail, T.A., Toth, A.M., Jarvis, D.L., Powers, A.M., and Olson, K.E. 2013. Bioluminescent imaging and histopathologic characterization of WEEV neuroinvasion in outbred CD-1 mice. PLoS One 8:e53462.
  13. Lin, C.-H. and Jarvis, D.L. 2013. Utility of temporally distinct baculovirus promoters for constitutive and baculovirus-inducible transgene expression in transformed insect cells. J. Biotechnol. 165:11-17.
  14. Serena, M.S., Geisler, C., Metz, G.E., Corva, S.G., Mórtola, E.C., Jarvis, D.L., Echeverría, M.G. 2013. Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs. Prot. Expr. Purif., 90:1-8.
  15. Gao, Y., Aryal, R.P., Ju, T., Cummings, R.D., Gahlay, G., Jarvis, D.L., Matta, K.L., Vlahakis, J.Z., Szarek, W.A., and Brockhausen, I. 2013. Acceptor specificities and selective inhibition of recombinant human Gal- and GlcNAc-transferases that synthesize core structures 1, 2, 3 and 4 of O-glycans. Biochim. Biophys. Acta, 1830:4274-4281.
  16. Gerken, T.A., Revoredo, L., Thome, J., Tabak, L.A., Clausen, H., Vester-Christensen, M.B., Jarvis, D.L., Gahlay, G.K., and Moremen, K.W. 2013. The lectin domain of the polypeptide GalNAc transferase family of glycosyltransferases (ppGalNAc T’s) acts as a switch directing glycopeptide substrate glycosylation in an N- or C- direction further controlling mucin-type O-glycosylation. J. Biol Chem. 288:19900-19914.
  17. An, Y., Rininger, J.A., Jarvis, D.L., Jing, X., Ye, Z., Aumiller, J.J., Eichelberger, M., and Cipollo, J.F. 2013. Comparative glycomics analysis of influenza hemagglutinin (H5N1) produced in vaccine-relevant cell platforms. J. Proteome Res. 12:3707-3720.
  18. Lin, C.-H., Kuo, C.-W., Jarvis, D.L., and Khoo, K.-H. 2013. Facile removal of high mannose structures prior to extracting complex type N-glycans from de-N-glycosylated peptides retained by C18 solid phase to allow more efficient glycomic mapping. Proteomics 14:87-92. PMC3926941.
  19. Phillips, A.T., Schountz, T., Toth, A.M., Rico, A.B., Jarvis, D.L., Powers, A.M., and Olson, K.E. 2013. Liposome-antigen-nucleic acid complexes protect mice from lethal challenge with Western and Eastern equine encephalitis viruses. J. Virol. 88:1771-1780. PMC3911585
  20. Jarvis, D.L. 2014. Recombinant Protein Expression in Baculovirus-Infected Insect Cells. Meth. Enzymol. 536:149-163. PMC in process.
  21. Mabashi-Asazuma, H., Kuo, C.-W., Khoo, K.-H. and Jarvis, D.L. 2014. A novel baculovirus vector for the production of non-fucosylated recombinant glycoproteins in insect cells. Glycobiology 24:325-340. PMC3919471.
  22. Toth, A.M., Kuo, C.-W., Khoo, K.-H., and Jarvis, D.L. 2014. A new insect cell glycoengineering approach provides baculovirus-inducible glycogene expression and increases human-type glycosylation efficiency. J. Biotechnol. 182-183:19-29. PMC4095979.
  23. Harrison, R.L. and Jarvis, D.L. 2014. Transforming lepidopteran insect cells for continuous recombinant protein expression. Meth. Mol. Biol., submitted for publication.
  24. Harrison, R.L. and Jarvis, D.L. 2013. Transforming lepidopteran insect cells for improved protein processing. Meth. Mol. Biol., submitted for publication.
  25. Geisler, C., Mabashi-Asazuma, H., and Jarvis, D.L. 2014. An overview and history of glycoengineering in insect expression systems. Meth. Mol. Biol., submitted for publication.

(Last update 09/05/14)

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