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Needle Gauge Table


ROUTES OF ADMINISTRATION, VOLUME, AND NEEDLE GAUGES FOR DRUG ADMINISTRATIONS1

The University of Wyoming provides the following as guidelines only for use by IACUC-approved faculty and staff

 

Species

PO

SC2

IM3

IP

IV (bolus)

IV (slow)4

Mouse

10 µl/g

20-22 gauge

Gavage needle

10 µl/g

Scruff, dorsum

≤23 gauge

Insufficient muscle mass; Not recommended

20 µl/g

≤21 gauge

5 µl/g

Lateral tail v. 27-30 gauge

20 µl/g

Lateral tail v. 27-30 gauge

Rat

10 µl/g

18-20 gauge

Gavage needle

10 µl/g

Scruff, dorsum

≤20 gauge

300 µl/site quadriceps m. ≤21 gauge

20 µl/g

≤21 gauge

5 µl/g

Lateral tail or saphenous v. ≤23 gauge

20 µl/g

lateral tail of saphenous v. ≤23 gauge

Rabbit

10 ml/kg

1 ml/kg

Scruff, dorsum

≤20 gauge

0.25 ml/kg

Quadriceps m.

≤20 gauge

5 ml/kg

≤20 gauge

2 ml/kg

Marginal ear v. ≤21 gauge

10 ml/kg

Marginal ear v. ≤21 gauge

Pig

10 ml/kg

1 ml/kg

Scruff, dorsum

≤18 gauge

0.25 ml/kg

Quadriceps m.

≤18 gauge

1 ml/kg

≤18 gauge

2.5 ml/kg

Ear v.

≤18 gauge

5 ml/kg

Ear v.

≤18 gauge

Bird

 

1-3% bdy wt pectoral, interscapular

inguinal folds

≤21 gauge

0.2 ml/100 g

Pectoral m.

≤25 gauge

Not applicable

 

 

 

                1Does not apply to polyclonal/monoclonal antibody production, or fluid replacement volumes
                2≤3 SC sites per day
                3≤2 IM sites per day
                45-10 minutes duration


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