Engineering Intracellular Sialylation Pathways is a method for producing glycoproteins having sialylated oligosaccharides. The compositions of the invention comprise enzymes involved in carbohydrate processing and production of nucleotide sugars, nucleotide sequences encoding such enzymes, and cells transformed with these nucleotide sequences. The compositions of the invention are useful in methods for producing complex sialylated glycoproteins in cells of interest including mammalian and insect cells. Also provided are cells of interest that have been recombinantly engineered to produce new forms of sialylated glycoproteins, higher concentrations of sialylated glycoproteins, and/or elevated concentrations of donor substrates required for sialylation, as well as kits for expression of sialylated glycoproteins.
This technology can be used in the biotechnology industry by creating a greater variety of recombinant glycol-products through carbohydrate engineering efforts.
Among the benefits of this technology are potentially lower biotechnology costs, increased variety of glycosylated products to be generated in heterologous hosts, enhanced value of heterologous expression systems and increased application of heterologous cell expression products such as vaccines, therapeutics and diagnostic tools.