This technology consists of a method for the simultaneous development of multiple bacterial detection assays based on a T4 bacteriophage system. A T4 reporter bacteriophage strain is used that carries several genetic mutations. This invention also consists of the development of an assay for the rapid detection of pathogenic bacteria. Such an assay is capable of producing accurate results from complicated samples containing mixtures of microorganisms.
This technology has broad applications for use in the detection of bacterial food borne pathogens. This technology can also be used to create multiple detection assays within a matter of weeks to months as opposed to years.
This method is far less labor intensive than existing methods, such as PCR and ELISA. This technology also includes a single-tube apparatus that employs the reporter bacteriophage platform in an assay that is simple and easy to perform, inexpensive and fast.