Modifying Insect Cell Glycosylation Pathways with Baculovirus Expression Vectors

The invention first provides a baculovirus expression vector characterized as either: (a) comprising at least a first and a second glycosylation enzyme transcription unit, the transcriptional units comprising a first and a second structural gene encoding a first and second oligosaccharide processing enzyme, each gene operatively positioned under the control of and in frame with a promoter; or (b) compromising at least a first glycosylation enzyme transcriptional unit, the transcriptional unit comprising a structural gene encoding an oligosaccharide processing enzyme, the gene operatively positioned under the control of a baculoviral immediate early, delayed early, early or late promoter.

Applications

The insect cells can be used for high volume, glycosylated protein production.

Features & Benefits

This cell line has normal morphologies, growth properties and remains competent as a host for baculovirus expression vectors. The cell line offers a distinct advantage over unmodified hosts for the baculovirus-meditated production of more authentic recombinant N-glycoproteins.