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Wyoming Research Products Center

 Modifying Insect Cell Glycosylation Pathways with Baculovirus Expression Vectors


Description of Technology:

The invention first provides a baculovirus expression vector characterized as either: (a) comprising at least a first and a second glycosylation enzyme transcription unit, the transcriptional units comprising a first and a second structural gene encoding a first and second oligosaccharide processing enzyme, each gene operatively positioned under the control of and in frame with a promoter; or (b) compromising at least a first glycosylation enzyme transcriptional unit, the transcriptional unit comprising a structural gene encoding an oligosaccharide processing enzyme, the gene operatively positioned under the control of a baculoviral immediate early, delayed early, early or late promoter.


The insect cells can be used for high volume, glycosylated protein production.

Features and Benefits:

This cell line has normal morphologies, growth properties and remains competent as a host for baculovirus expression vectors. The cell line offers a distinct advantage over unmodified hosts for the baculovirus-meditated production of more authentic recombinant N-glycoproteins.

For More Information Please Contact:

Davona Douglass, J.D.
(307) 766-2509

Tech ID


Patent Status

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