The invention first provides a baculovirus expression vector characterized as either: (a) comprising at least a first and a second glycosylation enzyme transcription unit, the transcriptional units comprising a first and a second structural gene encoding a first and second oligosaccharide processing enzyme, each gene operatively positioned under the control of and in frame with a promoter; or (b) compromising at least a first glycosylation enzyme transcriptional unit, the transcriptional unit comprising a structural gene encoding an oligosaccharide processing enzyme, the gene operatively positioned under the control of a baculoviral immediate early, delayed early, early or late promoter.
The insect cells can be used for high volume, glycosylated protein production.
This cell line has normal morphologies, growth properties and remains competent as a host for baculovirus expression vectors. The cell line offers a distinct advantage over unmodified hosts for the baculovirus-meditated production of more authentic recombinant N-glycoproteins.
Davona Douglass, J.D.Director(307) firstname.lastname@example.org
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