CELL PHYSIOLOGY SYLLABUS
ZOO 4670 (ZOO 5670)
Spring 2006
Disclaimer
(1) Some lectures deal with psychiatric disease.
(2) Lab session requires dissecting tissue from anesthetized rodents.
(3) Don�t register for this class if you are afraid of either (1) or
(2) above.
(4) Schedule and content of the lecture and lab session is subject to
change based on student�s progress toward achieving learning objectives.
INSTRUCTOR: � Dr.
TA������������������������������ Mr. Andrew Young
OFFICE:
BS 200B Office hours (by appointment)
Phone:
� 766 5558 (lab); 766 5602 (Office)
Email:
neuron@uwyo.edu
Course hours:
Lecture: M.W.F. 8.00 a. m. to
����������������������������������� � Lab: Thursday
Websites: ��������������� ���http://www.uwyo.edu/neuron/cellphysiology.htm
TEXTBOOK:
Cell and Molecular Biology: Concepts
and Experiments. 4th Edition.� Author: Gerald Karp. Publisher: Wiley.
Electronic handouts will also be available at the course website.
Credit hours:���������� � �4
Prerequisite:����������� �� Bio 2020 or instructor�s consent.
COURSE OBJECTIVES:
1. To study mammalian
cell functions and relate them to cell structure.
2. To gain an
understanding of modern experimental techniques used in cellular physiological
research
3. To gain critical
thinking and integrative writing skills required for cellular and physiological
research
COURSE OUTLINE:
1. Overview and review
of basic cell structure function and cell diversity in mammals
2. Membrane voltage-gated
ion channels: molecular structure, biophysics and regulation of physiological
process
3. Transport across
biological membranes: molecular structures, biophysics and regulation of
physiological process
4. Cell-to-cell
signaling: hormones, receptors and intracellular messengers
5. Protein synthesis, vesicular trafficking, endocytosis
and exocytosis
7. Neurons, synaptic
transmission and ligand-gated ion channels
8. Extracellular matrix
and cell-cell interactions
9. Genetic approaches to
study the physiology of mammalian cell
10. Aspects of
experimental techniques in cell biology and cell physiology
EVALUATION AND GRADING:
For all students: 3 in-course exams (25% each), attendance and labs (10%). For
Undergraduate students:� term presentation
(15%). For graduate students: term assay (15%). Assignments may also be given
and used in evaluation within the 10% or as part of an exam. There will be
three equally-weighted scheduled in-course exams. The in-course exams will
consist of 10-20 multiple-choice questions, data interpretation questions and
short assay questions. Evaluation is ongoing to enhance learning, providing the
student with feedback about performance in meeting the course objectives.
Grading is a process of measuring the outcome of learning against standards and
assigning a symbol to the level of performance achieved. The final course
grade, therefore, rests with the professor. Grading scale: 90% and above-A, 80%
to 89%-B, 70%to 79%-C,60% to 69%-D, below 60%-F.
**All students are expected
to conform to college-level standards of ethics, academic integrity, grammar
and spelling.� Except in cases where
prior arrangements have been made with the instructor, there is
no provision for making up late work and/or missed quizzes or exams.
CELL PHYSIOLOGY
ZOO 4670 (also ZOO 5670)
Fall 2006
TENTATIVE CLASS SCHEDULE (check the schedule and handout link in
th
Please note that this
schedule is subject to changes, which will be announced in class.
Responsibility to keep up with changes, assignments etc. lies with the student.
Additional topics will be covered as time permits.
|
Week |
Topic |
Text Chapter(s) and
recommended reading |
|
1 (Aug-30-W.) |
Overview and review
of basic cell structure, function and cell diversity in mammals |
Chapter 1 |
|
September-04-M |
Labor day
no class |
|
|
2 (September-06-W) |
Membrane
voltage-gated ion channels (part 1): molecular structure, biophysics |
Ch 2.1,-2.5; Ch4 |
|
3 (September-11-M) |
Membrane
voltage-gated ion channels (part 2): regulation of physiological and
pathological process |
Ch4 |
|
4 (September-18-M) |
Microscopy theory
and techniques, novel microscopy techniques |
Ch18 |
|
5 (September-25-M) |
Transport across
biological membranes: molecular structures, biophysics and regulation of
physiological and pathological process |
Ch4.5-4.7 |
|
6
(October-02-M) |
Exam 1 :
Biological membrane and novel microscopy techniques |
|
|
7� (October-9-M) |
Cell-to-cell
signaling: hormones, receptors and intracellular messengers (part 1) |
Ch15 |
|
October
16-18 M.W. |
Society
for Neuroscience Conference, no class |
|
|
8 October-23-M |
Cell-to-cell
signaling (2) : physiology and disease |
Ch15 |
|
9 October-30-M |
Protein synthesis,
vesicular trafficking, endocytosis and exocytosis |
Ch8 |
|
10 November-6-M |
Extracellular matrix
and cell-cell interactions |
Ch7 |
|
11 November-10-F |
Exam 2:
Intracellular machinery and cell-cell interaction |
|
|
12
November-13-M |
Neurons and glia |
N.A. |
|
13 November-20-M |
Synaptic
transmission, Ligand-gated ion channels, and
neuromuscular junction |
N.A. |
|
22-24 |
Happy
Thanks Giving. No Class |
|
|
14
November-27-M |
Genetic approaches
to study the physiology of mammalian cell: |
Ch 10- Ch12 |
|
15
December-04-M |
Aspects
of experimental techniques in cell physiology |
Ch18 |
|
16
December-08-M |
Student
Presentations Last day
of class |
|
|
17 December-11-M |
Final exam
(Graduate
students turn-in term assays) |
|
TENTATIVE LAB SCHEDULE
|
Week |
Topics |
�Location see below |
|
Week 1-3* |
No lab |
� |
|
Lab 1 (Week 4) September-19-T |
Nobel lecture No.1 Roderick McKinnon: Potassium channels |
TBA |
|
Lab 2 (group 1, week
5) September-28-T |
Understanding
membrane potentials and propagation of action potentials using computer
simulations (1); |
Computer lab |
|
Lab 2 (Group 2, week
6) October-5-TR |
Understanding
membrane potentials and propagation of action potentials using computer
simulations (2); |
Computer lab |
|
Lab 3 (Week 7) October-10-T |
Paul Greengard � Nobel Lecture The Neurobiology of
Dopamine Signaling |
TBA |
|
October
16-19 M.W. |
Society
for Neuroscience Conference, no lab |
|
|
Lab 4
(week 8, group 1) October-24-T |
Dissecting nerve tissue, isolating neurons from rat brain (1) |
BS218 |
|
Lab 4 (week 9, group
2) November-2-TR |
Dissecting nerve tissue, isolating neurons from rat brain (1) |
BS218 |
|
Lab 5
(week 10, group 1) November-7-T |
Obtaining brain
slices from GFP mice /live visualization/Fixation and immunostaining
(1) |
BS218 |
|
Lab 5 (week 11,
group 2) November-16-TR |
Obtaining brain
slices from GFP mice/live visualization/Fixation and immunostaining
(2) |
|
|
November-
22-24 (WF) |
Happy
Thanks Giving. No Lab |
|
|
Lab 6
(week 12, both groups) November-28-T |
presentation
1 |
TBA |
|
Lab 7 (week 13, both
groups) December-7-TR |
� presentation2 |
BS218 |
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