THE VIRTUAL EDGE: Lab 5 Cultivation of Bacteria I

Procedure: (work in pairs)Collect 1 GSA plate, 1 TSA plate, and 1 EMB plate.

Organisms that you will be working with:

Escherichia coli (G-)
Staphylococcus epidermidis (G+)
Pseudomonas aeruginosa (G-)
Enterobacter aerogenes (G-)

  1. With a marker, divide the bottom of all three Petri plates into quadrants.  Label the plates with your name, lab section, and the date.  Label each quadrant with one of the four organisms as shown in the diagram below.
  2. Follow the procedure for Innoculating organisms into four quadrants.
  3. Repeat the procedure for all four organisms.  Streak them in their appropriately labeled quadrants.
  4. Repeat the inoculation procedure, as described in the video, for the remaining 2 plates. 
  5. Invert the plates and incubate them at 37°C for 24-48 hours.

Lab 5 / Aseptic Technique / Nutritional Requirements for the Cultivation of Bacteria / Enumeration of Bacteria / Dilutions / Lab 5 Organisms

Please take a few minutes to fill out a brief survey about your experience using the Virtual Edge: https://docs.google.com/forms/d/1yGbkF0KM92WBSk-IgS-EkjxkTKTQwhzuXmDsVpwRDoU/viewform

Please email comments/problems to cboggs@uwyo.edu

Rachel Watson, M.S.
AG 5010
766-3524
Cell: 307-760-2942
rwatson@uwyo.edu

Microbiology Lecture WebsiteMicrobiology Lab WebsiteThe Virtual Edge Homepage

Creative Commons License
The Virtual Edge by http://www.uwyo.edu/virtual_edge/ is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 United States License