Extra Credit for Unknown Report:

Instructions for using GenBank:

1. Click on your unkown number and download the 16S rRNA sequence. This is for your gram- unknown only.
   

   21	100	188	276
   2	101	189	277
   3	102	190	278
   4	103	191	279
   5	104	192	280
   6	105	193
   7	106	194
   8	107	195
   9	108	196
   10	109	197
   11	110	198
   12	111	199
   13	112	200
   14	113	201
   15	114	202
   16	115	203
   17	116	204
   18	117	205
   19	118	206
   20	119	207
   21	120	208
   22	121	209
   23	122	210
   24	123	211
   25	124	212
   26	125	213
   27	126	214
   28	127	215
   29	128	216
   30	129	217
   31	130	218
   32	131	219
   33	132	220
   34	133	221
   35	134	222
   36	135	223
   37	136	224
   38	137	225
   39	138	226
   40	139	227
   41	140	228
   42	141	229
   43	142	230
   44	143	231
   45	144	232
   46	145	233
   47	146	234
   48	147	235
   49	148	236
   50	149	237
   51	150	238
   52	151	239
   53	152	240
   54	153	241
   55	154	242
   56	155	243
   57	156	244
   58	157	245
   59	158	246
   60	159	247
   61	160	248
   62	161	249
   63	162	250
   64	163	251
   65	164	252
   66	165	253
   67	166	254
   68	167	255
   69	168	256
   70	169	257
   71	170	258
   72	171	259
   73	172	260
   74	173	261
   75	174	262
   76	175	263
   77	176	264
   78	177	265
   79	178	266
   80	179	267
   81	180	268
   82	181	269
   83	182	270
   84	183	271
   85	184	272
   86	185	273
   87	186	274
   88	187	275

2. Copy the sequence.
3. To search GenBank for sequence matches type in the url: http://www.ncbi.nih.gov/
4. On the side bar select the icon entitled GenBank.
5. On the top bar select the icon BLAST.
6. Under the large heading entitled BLAST Assembled Genomes, click on the icon entitled microbes.
7. Enter your query sequence in the space provided.
8. Below the query sequence section there is a list of Domains. Scroll down and select Bacteria by hitting the + sign.
9. Scroll back up and hit the BLAST icon to begin your search.
10. On the next screen a request ID number will be displayed, to the right of that click on View report.
11. When the results screen appears, scroll down until you see the numbers in blue text.
12. The top listed bacterium is the one with the greatest sequence match to your unknown. In order to recieve full extra credit points, write down the full line of text including the reference #, genus and species name and score of the best sequence match. Does this match agree with your phenotypic identification?

Rachel Watson, M.S.
AG 5010
766-3524
Cell: 307-314-9636
rwatson@uwyo.edu