GENERAL MICROBIOLOGY LABORATORY
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Fall Semester 2007
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| Day |
Date |
Lab # |
Lab Exercises |
| W/Th |
Jan. 14/15 |
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Introduction and Pretest |
| M/T |
Jan. 19/20 |
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NO LAB |
| W/Th |
Jan. 21/22 |
1 |
Ubiquity of Microorganisms |
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Microscopy |
| M/T |
Jan. 26/27 |
2 |
Simple Stains: Direct and Negative |
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Morphological Unknown: Direct stain |
| W/Th |
Jan. 28/29 |
3 |
Differential Stains: Cell Wall (Gram and Acid-Fast) |
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Morphological Unknown: Gram and Acid-Fast stain |
| M/T |
Feb. 2/3 |
4 |
Differential Stains: Cell Structures (Endospore and Capsule) |
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Morphological Unknown: Endospore Stain |
| W/Th |
Feb. 4/5 |
5 |
Aseptic Technique |
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Cultivation of Bacteria: Culture Media/Nutritional Requirements |
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Enumeration of Bacteria: Standard Plate Count |
| M/T |
Feb. 9/10 |
6 |
Cultivation of Bacteria: Pure Culture/Streak Plate |
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Environmental Influences: Temp., Osmotic Pressure and Oxygen |
| W/Th |
Feb. 11/12 |
7 |
Microbial Diversity and Ecology: Bioluminescence and the Winogradsky
Column (Pipetmen) |
| M/T |
Feb. 16/17 |
8 |
Control of Microbial Growth: UV and Antibiotics |
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Bacterial Genetics: Isolation of Drug Resistant Mutants |
| W/Th |
Feb. 18/19 |
9 |
Control of Microbial Growth: Disinfectants and Antiseptics |
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Effectiveness of Antiseptics in Hand Washing |
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Bacterial Genetics: Isolation of Drug Resistant Mutants (cont.) |
| M/T |
Feb. 23/24 |
10 |
Bacterial Genetics: Transformation |
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11 |
Bacteriophage: Isolation |
| W/Th |
Feb. 25/26 |
12 |
Bacteriophage: Titering a Phage Suspension |
| M/T |
Mar. 2/3 |
13 |
Fungi |
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Lab Practical Review |
| W/Th |
Mar. 4/5 |
14 |
Practical Exam I |
| M/T |
Mar. 9/10 |
15 |
Enzyme-Mediated Biochemical Activities of Bacteria: Physiological Testing
for Identification |
| W/Th |
Mar. 11/12 |
16 |
Enzyme-Mediated Biochemical Activities of Bacteria: Physiological Testing
for Identification |
| M/T |
Mar. 16/17 |
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Spring Break |
| W/Th |
Mar. 18/19 |
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Spring Break |
| M/T |
Mar. 23/24 |
17 |
Complete Physiological Testing for Identification |
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Unknown: Gram Stain and Streak for Isolation |
| W/Th |
Mar. 25/26 |
18 |
Unknown: Gram Stain and Prepare Reserve and Working Stocks |
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Food Microbiology: Preparation of Wine |
| M/T |
Mar. 30/31 |
19 |
Unknown: Biochemical Testing |
| W/Th |
Apr. 1/2 |
20 |
Unknown: Biochemical Testing |
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The Rapid KOH String Test |
| M/T |
Apr. 6/7 |
21 |
Genomics and the Application of Computer-Based Nucleotide
Sequence Searches for Identification of Unknown Bacteria and their
Phylogenetic Relationships |
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Complete Unknown: Identification |
| W/Th |
Apr. 8/9 |
22 |
Immunology |
| M/T |
Apr. 13/14 |
23 |
Water Microbiology: PA test, Membrane Filtration and MTFM |
| W/Th |
Apr. 15/16 |
24 |
Soil Microbiology: Enrichment of Azotobacter and Enumeration |
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Water Microbiology (cont.) |
| M/T |
Apr. 20/21 |
25 |
Food Microbiology: Natural Antimicrobials in Food |
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Soil Microbiology: Nitrogen Fixation (cont.) and Ammonification |
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Water Microbiology (cont.) |
| W/Th |
Apr. 22/23 |
26 |
Food Microbiology: Preparation of Yogurt (clean up) |
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Lab Practical Review |
| M/T |
Apr. 27/28 |
27 |
Practical Exam II |
