Gram-positive unknown: Culture A
_____ � Perform a Gram stain (Gram Stain Procedure) using growth from the working slant. This should be done first! If there is any sign of contamination it will be necessary to inoculate a fresh stock before proceeding.
_____ � Inoculate one tube of TSB using an inoculating loop.
_____ � Inoculate one tube of Nitrate broth using an inoculating loop. (Nitrate Test Procedure) (Nitrate Test Results)
_____ � Inoculate motility agar using a needle to make one stab down the center to the bottom of the tube.
(Stab Procedure)
_____ � Perform the catalase test.(Catalase Test)
USE A WOODEN STICK -- NOT A LOOP!!!
_____ � If your unknown is a Gram-positive coccus, streak one plate of Blood Agar. T-streak using the entire plate; use the inoculating loop to make several stabs in a streak-free region of the agar.
_____ � If your unknown is a Gram-positive rod, inoculate one Nutrient Agar + MnCl2 slant for a future spore stain
(Use an inoculating loop and streak the surface of the slant).
_____ � If your unknown is a Gram-positive rod, perform an acid-fast stain. (Acid-fast Stain Procedure)
