THE VIRTUAL EDGE: Labs 19-21 Identification of Bacterial Unknowns

CHECKLIST 3

Gram-positive unknowns: Culture A

_____ - For Gram-positive rods, perform a spore stain from the culture grown on the Nutrient Agar + MnCl2 slant. (Endospore Stain Procedure)
_____ For Gram-positive rods, flood the starch side of the starch and spirit blue plate with iodine. Add just enough iodine to cover the surface of the starch agar and allow the plate to sit for 5 to 10 minutes before observing.

Gram-negative unknown: Culture B

_____ - Add 6 to 7 drops of Kovac�s reagent to the top of the SIM tube. (SIM Results Procedure)
_____ - Perform the MR-VP Test:

  1. Remove 1, 0.5 mL aliquot from the unknown MR-VP culture and place it into a test tube.
  2. Label the tube containing 0.5 mL, MR and the tube containing the remainder of the culture, VP.

Labs 19-21 / Interactive Dichotomous Key / Summary of Biochemical Tests / Virtual Edge Home

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