Temperature Procedure:

All 4 microorganisms will be tested at all 4 temperatures.
Temperatures: 4˚C, 22˚C (room temp), 37˚C, and 55˚C.
Test Organisms:

Pseudomonas fluorescens
Escherichia coli
Bacillus stearothermophilus
Bacillus subtilis

1. Collect 4 TSA plates from the side bench.  On the bottom of the plates use a marker to divide them into 4 quadrants as shown below.
   
2. Follow the procedure for Innoculating organisms into four quadrants.
3. Repeat the procedure for all four organisms.  Streak them in their appropriately labeled quadrants.
4. Repeat the inoculation procedure, as described in the video, for the remaining 3 plates. 
5. Invert and incubate the plates in the appropriate temperature incubators for 24 - 48 hours.  Place the plate that will be incubated at 55 ˚C into a plastic container with a moist towel to prevent dehydration.

Rachel Watson, M.S.
AG 5010
766-3524
Cell: 307-760-2942
rwatson@uwyo.edu