The Virtual Edge

THE VIRTUAL EDGE: Lab 8 Control of Microbial Growth I

Antimicrobial Sensitivity

Kirby Bauer Technique

Many soil bacteria and some fungi secrete substances, called antibiotics that inhibit the growth of other bacteria. Paper discs impregnated with different antibiotics can be placed on a special agar medium (Mueller-Hinton) that has been spread with a particular strain of bacteria. After incubation a bacterial lawn will arise on the agar medium. If the strain is sensitive to the antibiotic in the disc there will be clear zone of inhibition (no bacterial growth) around the disc. This type of procedure allows physicians to prescribe appropriate and effective antibiotics for patients.

Different antibiotics "work" by attacking certain targets on or in bacteria. Because different antibiotics have different targets, they are selectively toxic. That is, certain organisms can be killed by an antibiotic while others or only inhibited or not affected at all.

Target or mechanism	Example of an antibiotic with this target or mechanism	Often used to treat
Bacterial cell wall	penicillin G	Gram-positive bacteria*
Inhibit protein synthesis	tetracycline, streptomycin, erythromycin and gentamicin	A broad-spectrum of bacteria
Bacterial cell membrane	nystatin and polymyxin	polymyxin-Gram-negative bacteria nystatin-fungal infections
Affect DNA or RNA synthesis	naladixic acid	A broad-spectrum of bacteria
Antimetabolite	sulfa drugs (sulfonamides) (considered antibiotics even though they are synthetic agents and not microbially synthesized)	A broad-spectrum of bacteria*
*Because of improper use and overuse, an increasing number of bacteria are becoming resistant.

Terms and Definitions

Antibiotic: a substance produced by one living organism that kills or inhibits the growth of another organism.
Bactericide: a substance that kills bacteria.
Bacteriostatic: a substance that inhibits the growth and reproduction of bacteria.
Antimicrobic: any agent that kills or inhibits the growth of microorganisms.

Lab 8 / Lethal Effects of Ultraviolet Radiation / Antimicrobial Sensitivity: Kirby Bauer Technique / Isolation of Drug Resistant Mutants / Lab 8 Organisms

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