Fungi Procedure

1. Ascomycota

Make a wet mount slide of Saccharomyces cerevisiae using the following procedure:

    • Place 3-4 loopfuls of water on the slide.
    • Flame an inoculating loop and allow it to cool.  Pick up a single colony of Saccharomyces cerevisiae and mix it into the drop of water on the slide.
    • Place a cover slip over the mixture.  Be careful to avoid bubbles.

Observe the slide under high dry and oil immersion.  Try to identify the budding cells.  Make a sketch in the results section.

Observe the colonies of Aspergillus niger and Penicillium camembertii. Describe the morphology and draw an example of each colony in the results section. View the plates under the dissecting microscope. Make a sketch and try to identify the hyphae and buds. DO NOT REMOVE THE PLATES’ LIDS.

2. Basidiomycota

Observe the oyster, shitake and portabella mushrooms.  Note the gills on the underside of the cap.  Make a wet mount of a portabella gill using the following procedure:

    • Using a razor blade, cut out a portion of a single gill and place it onto a microscope slide. 
    • Place several drops of sterile water onto the gill and cover it with a cover slip. 

Observe this slide under the low power and high dry objective lenses.  Make a sketch in the results section.  Be sure to label the basidia covering the gill.

3. Zygomycota

Observe the colonies of Rhizopus stolonifer.  Describe the morphology and draw an example of a colony in the results section.  View the plate under the dissecting microscope.  Make a sketch and try to identify the hyphae and sporangia filled with black spores.  DO NOT REMOVE THE PLATE’S LID.

Lab 13 / Fungi / Classification of Fungi / Procedure / Results / Lab 13 Organisms

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