Laser Scanning Confocal Microscope (ZEISS 700)
· Motorized upright microscope (Imager.Z2)· Objectives include a 5X, 10X, 20X, 40X (oil), and 63X (oil)
· Four laser lines (all solid state): 405, 488, 555, and 639 nm.
· Two regular fluorescence PMTs and one transmitted light PMT.
Emission filters used in conjunction with the variable secondary dichroic (VSD) beamsplitter
| PMT 1 | PMT 2 |
|---|---|
|
490-555nm |
505-600nm |
| 490-635nm | LP 490nm |
| SP 490nm | LP 560nm |
| SP 555nm | LP 615nm |
| SP 640nm | LP 640nm |
| 465-505nm + LP 525nm | 555-610nm + LP 650nm |
| 420-470nm + 500-610nm | 495-535nm + LP 555nm |
