Laser Scanning Confocal Microscope (ZEISS 700)

· Motorized upright microscope (Imager.Z2)

· Objectives include a 5X, 10X, 20X, 40X (oil), and 63X (oil)

· Four laser lines (all solid state): 405, 488, 555, and 639 nm.

· Two regular fluorescence PMTs and  one transmitted light PMT.

Emission filters used in conjunction with the variable secondary dichroic (VSD) beamsplitter

PMT 1	PMT 2
490-555nm	505-600nm
490-635nm	LP 490nm
SP 490nm	LP 560nm
SP 555nm	LP 615nm
SP 640nm	LP 640nm
465-505nm + LP 525nm	555-610nm + LP 650nm
420-470nm + 500-610nm	495-535nm + LP 555nm