Department of Chemistry, University of Wyoming


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Book chapters:

[1]        F. Basile, Mignon, Rudolph K., Methods and Instrumentation in Mass Spectrometry for the Differentiation of Closely Related Microorganisms, in: Appl. Mass Spectrom. Microbiol. Strain Charact. Rapid Screen. Antibiot. Resist., Springer, 2016: pp. 13–50.

[2]        F. Basile, Rapid Sample Preparation for Microorganism Analysis by Mass Spectrometry, in: C. Fenselau, P. Demirev (Eds.), Rapid Charact. Microorg. Mass Spectrom., American Chemical Society, Washington, DC, 2011: pp. 5–34.


Peer-reviewed manuscripts:

[3]        R. Zhou, F. Basile, Plasmonic Thermal Decomposition/Digestion of Proteins: A Rapid On-Surface Protein Digestion Technique for Mass Spectrometry Imaging, Anal. Chem. 89 (2017) 8704–8712. doi:10.1021/acs.analchem.7b00430

[4]        R. Zhou, F. Basile, Convective-heating thermal decomposition/digestion of peptides and proteins on surfaces, J. Anal. Appl. Pyrolysis. (2017). In Press, doi:10.1016/j.jaap.2017.07.004.

[5]        T.F. Kinde, T.D. Lopez, F. Basile, D. Dutta, Correction to Electrophoretic Extraction of Low Molecular Weight Cationic Analytes from Sodium Dodecyl Sulfate Containing Sample Matrices for Their Direct Electrospray Ionization Mass Spectrometry, Anal. Chem. 89 (2017) 2180–2180. doi:10.1021/acs.analchem.7b00102.

[6]        T.F. Kinde, F. Basile, D. Dutta, Correction to A Microfluidic SPLITT Device for Fractionating Low-Molecular Weight Samples, Anal. Chem. 89 (2017) 2179–2179. doi:10.1021/acs.analchem.7b00101.

[7]        R.K. Mahat, C. Seebart, F. Basile, N. Ward, Global and targeted lipid analysis of Gemmata obscuriglobus reveals the presence of lipopolysaccharide, a signature of the classical Gram-negative outer membrane, J Bacteriol. 198 (2016) 221–236. doi:10.1128/JB.00517-15.

[8]        C. Liu, E. Topchiy, T. Lehmann, F. Basile, Characterization of the dehydration products due to thermal decomposition of peptides by liquid chromatography-tandem mass spectrometry, J. Mass Spectrom. 50 (2015) 625–632. doi:10.1002/jms.3570.

[9]        R.K. Mahat, W. Rodgers, F. Basile, Microwave Radiation Heating in Pressurized Vessels for the Rapid Extraction of Coal Samples for Broad Spectrum GC–MS Analysis, Energy Fuels. 28 (2014) 6326–6335. doi:10.1021/ef501659h.

[10]      L. Lu, F. Basile, Thermal decomposition of phosphorylated peptides in the condensed phase as studied by mass spectrometry, J. Anal. Appl. Pyrolysis. 104 (2013) 412–417. doi:10.1016/j.jaap.2013.06.004.

[11]      G.M. Toh-Boyo, S.S. Wulff, F. Basile, Comparison of Sample Preparation Methods and Evaluation of Intra- and Intersample Reproducibility in Bacteria MALDI-MS Profiling, Anal. Chem. 84 (2012) 9971–9980. doi:10.1021/ac302375e.

[12]      C. Liu, F. Basile, Intermolecular condensation products formed during the pyrolysis of peptides, J. Anal. Appl. Pyrolysis. 92 (2011) 217–223. doi:10.1016/j.jaap.2011.05.011.

[13]      F. Basile, S. Zhang, S.K. Kandar, L. Lu, Mass Spectrometry Characterization of the Thermal Decomposition/Digestion (TDD) at Cysteine in Peptides and Proteins in the Condensed Phase, J. Am. Soc. Mass Spectrom. 22 (2011) 1926–1940. doi:10.1007/s13361-011-0222-9.

[14]      F. Basile, T. Sibray, J.T. Belisle, R.A. Bowen, Analysis of lipids from crude lung tissue extracts by desorption electrospray ionization mass spectrometry and pattern recognition, Anal. Biochem. 408 (2011) 289–296. doi:10.1016/j.ab.2010.09.017.

[15]      F. Basile, N. Hauser, Rapid Online Nonenzymatic Protein Digestion Combining Microwave Heating Acid Hydrolysis and Electrochemical Oxidation, Anal. Chem. 83 (2011) 359–367. doi:10.1021/ac1024705.

[16]      F. Basile, S. Zhang, Y.-S. Shin, B. Drolet, Atmospheric pressure-thermal desorption (AP-TD)/electrospray ionization-mass spectrometry for the rapid analysis of Bacillus spores, The Analyst. 135 (2010) 797. doi:10.1039/c0an00071j.

[17]      N.J. Hauser, H. Han, S.A. McLuckey, F. Basile, Electron Transfer Dissociation of Peptides Generated by Microwave D-Cleavage Digestion of Proteins, J. Proteome Res. 7 (2008) 1867–1872. doi:10.1021/pr700671z.

[18]      N.J. Hauser, F. Basile, Online Microwave D-Cleavage LC-ESI-MS/MS of Intact Proteins: Site-Specific Cleavages at Aspartic Acid Residues and Disulfide Bonds, J. Proteome Res. 7 (2008) 1012–1026. doi:10.1021/pr700596e.

[19]      S. Zhang, Y.-S. Shin, R. Mayer, F. Basile, On-probe pyrolysis desorption electrospray ionization (DESI) mass spectrometry for the analysis of non-volatile pyrolysis products, J. Anal. Appl. Pyrolysis. 80 (2007) 353–359. doi:10.1016/j.jaap.2007.04.005.

[20]      S. Zhang, F. Basile, Site-Specific Pyrolysis-Induced Cleavage at Aspartic Acid Residue in Peptides and Proteins, J. Proteome Res. 6 (2007) 1700–1704. doi:10.1021/pr060648w.

[21]      Y.-S. Shin, B. Drolet, R. Mayer, K. Dolence, F. Basile, Desorption Electrospray Ionization-Mass Spectrometry of Proteins, Anal. Chem. 79 (2007) 3514–3518. doi:10.1021/ac062451t.

[22]      M.A. Meetani, Y.-S. Shin, S. Zhang, R. Mayer, F. Basile, Desorption electrospray ionization mass spectrometry of intact bacteria, J. Mass Spectrom. 42 (2007) 1186–1193. doi:10.1002/jms.1250.

[23]      F. Basile, G.E. Kassalainen, S.K. Ratanathanawongs Williams, Interface for Direct and Continuous Sample−Matrix Deposition onto a MALDI Probe for Polymer Analysis by Thermal Field Flow Fractionation and Off-Line MALDI-MS, Anal. Chem. 77 (2005) 3008–3012. doi:10.1021/ac048391d.

[24]      C.D. Havey, F. Basile, C. Mowry, K.J. Voorhees, Evaluation of a micro-fabricated pyrolyzer for the detection of Bacillus anthracis spores, J. Anal. Appl. Pyrolysis. 72 (2004) 55–61. doi:10.1016/j.jaap.2004.02.002.

[25]      K.Y.C. Fung, S. Askovic, F. Basile, M.W. Duncan, A simple and inexpensive approach to interfacing high-performance liquid chromatography and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry, Proteomics. 4 (2004) 3121–3127. doi:10.1002/pmic.200300843.

[26]      M.A. Meetani, F. Basile, K.J. Voorhees, Investigation of pyrolysis residues of poly(amino acids) using matrix assisted laser desorption ionization-time of flight-mass spectrometry, J. Anal. Appl. Pyrolysis. 68–69 (2003) 101–113. doi:10.1016/S0165-2370(03)00070-6.

[27]      F. Basile, A Gas Sample Pre-concentration Device Based on Solid Phase Microextraction (SPME) and Temperature Programmed Desoprtion (TPD), Instrum. Sci. Technol. 31 (2003) 155–164.

[28]      F. Basile, I. Ferrer, E.T. Furlong, K.J. Voorhees, Simultaneous Multiple Substrate Tag Detection with ESI-Ion Trap MS for In Vivo Bacterial Enzyme Activity Profiling, Anal. Chem. 74 (2002) 4290–4293. doi:10.1021/ac020249u.

[29]      A.J. Madonna, F. Basile, E. Furlong, K.J. Voorhees, Detection of bacteria from biological mixtures using immunomagnetic separation combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Rapid Commun. Mass Spectrom. 15 (2001) 1068–1074. doi:10.1002/rcm.344.

[30]      A.J. Madonna, F. Basile, I. Ferrer, M.A. Meetani, J.C. Rees, K.J. Voorhees, On-probe sample pretreatment for the detection of proteins above 15 KDa from whole cell bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Rapid Commun. Mass Spectrom. 14 (2000) 2220–2229.;2-4/full (accessed November 28, 2013).

[31]      F. Basile, M.B. Beverly, K.J. Voorhees, T.L. Hadfield, Pathogenic bacteria: their detection and differentiation by rapid lipid profiling with pyrolysis mass spectrometry, TrAC Trends Anal. Chem. 17 (1998) 95–109. (accessed November 28, 2013).

[32]      F. Basile, M.B. Beverly, C. Abbas-Hawks, C.D. Mowry, K.J. Voorhees, T.L. Hadfield, Direct mass spectrometric analysis of in situ thermally hydrolyzed and methylated lipids from whole bacterial cells, Anal. Chem. 70 (1998) 1555–1562. (accessed November 28, 2013).

[33]      F. Basile, K.J. Voorhees, T.L. Hadfield, Microorganism gram-type differentiation based on pyrolysis-mass spectrometry of bacterial Fatty Acid methyl ester extracts., Appl. Environ. Microbiol. 61 (1995) 1534–1539. (accessed December 5, 2013).

[34]      F. Basile, K.D. Hughes, P.E. Wisniowski, D.G. Gorenstein, F.E. Lytle, T.S. McCay-Buis, D.M. Huber, B.C. Hemming, Fast and Sensitive Laser-Based Enzymatic Detection of the Lactose Operon in Microorganisms, Anal. Biochem. 211 (1993) 55–60.


Dissertations from the Basile group: search at UW library

Poster presentations:

ASMS 2017  Characterization of the Lipid A Forms in Gemmata obscuriglobus by MALDI-ToF/ToF-MS, Rudolph Mignon ; Anthony Maus ; Rajendra Mahat ; Corrine Seebart ; Naomi Ward ; Franco Basile*, University of Wyoming, Laramie, WY

ASMS 2016   Differentiation of bacteria at the strain level by MALDI-MS of proteins >15kDa; Franco Basile* ; Bledar Bisha, Anthony Maus ,
University of Wyoming, Laramie, WY

ASMS2015  Code: MP05

(190) Liquid Chromatography Direct Electron Ionization Mass Spectrometry for the Analysis of Polycyclic Aromatic Hydrocarbons
Mitchell Helling; Franco Basile; University of Wyoming, Laramie, WY
ASMS2014  Code: TP32 Time Slot/Poster Number: 606 Session: Energy: Hydrocarbon and Petrochemical
(1683) Pressurized Heating for the Rapid Preparation/Extraction of Coal Samples for Broad Spectrum GC-MS Analysis
Franco Basile1; Rajendra Mahat1; Wesley Rodgers2; 1University of Wyoming, Laramie, WY; 2JR Simplot Co, Boise, ID

ASMS2014  Code: WP01   Session: Imaging MS: Sample Preparation

(1845) Photo-Thermal Decomposition/Digestion (Photo-TDD) of Proteins and its Application in MALDI-MS Imaging
Rong Zhou; Franco Basile; University of Wyoming, Laramie, WY

ASMS2013  Code: ThP25   Session: Microorganisms: Identification and Characterization
(2977) Differentiationof Borrelia species with MALDI-MS using spray sample deposition
Franco Basile1; Gwendoline Toh-Boyo1; Shaun Wulff1; Claudia Molins2; 1University of Wyoming, Laramie, WY; 2Centers for Disease Control and Prevention, Fort Collins, CO

ASMS2012  Code: WP09   Session: Microorganisms: Identification and Characterization

(2035) Evaluation of MALDI Intra Spot and Inter Sample Reproducibility in Bacteria Profiling with MALDI-MS
Franco Basile; Gwendoline Toh-Boyo; Shaun Wulff; University of Wyoming, Laramie, WY

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