Bacteriophage Linked Immunosorbent Assay for Rapid, Sensitive Detection of Multiple Analytes
Description of Technology:
This technology is a method for the development of a platform that enables the efficient and simultaneous creation of multiple detection assays. This technology enables the use of any bacteriophage, modified to carry a reporter gene, to which any analyte-recognizing moiety is attached via the capsid. The modified bacteriophage is used to attach the target analyte. This attachment is identified via bacteriophage amplification in a helper bacteria strain with a subsequent production and detection of the reporter gene.
The Bacteriophage Linked Immunosorbent Assay (BASILA) can be used in both a field setting (by employing the colorimetric substrate) or in a laboratory setting (by employing the fluorescent or luminescent substrates).
Features and Benefits:
The major advantage of this technology is sensitivity, as 10-100 bacteriophages would be able to produce a detectable signal. This technology can be produced on a standardized platform and is cost effective. Additionally this technology is versatile and can be used with or without instrumentation for both field and laboratory use.
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