Basile Lab

Lab and instrumentation

Biodetection and Mass Spectrometry Laboratory

Located in the Physical Sciences Bldg Room 444.

(Mass Spectrometry Instrumentation Lab is located in the Physical Sciences Bldg room 317)

Our Biodetection and Mass Spectrometry Laboratory has adjacent offices for graduate students.  We are equipped with instrumentation and computers to conduct a wide range of analytical MS measurements and data analyses.  Our laboratory also has an in-house microbiology lab (Biosafety Level 2) where we maintain and produce a wide range of bacterial and viral samples for MS biodetection research.

---

Q-Exactive-HF X a LC-MS/MS high resolution system based on the Orbitrap mass analyzer

Funded by NSF-EPSCoR RII Track-1: Linking microbial life to ecosystem services across Wyoming's dynamic landscape

 

Orbitrap-MS (Q-Exactive HF-X)  with U-HPLC RP (nano, capillary and micro flows)

nanoESI, heated-ESI (HESI) and APCI sources.

GC-High resolution (ToF) MS  (HRT+; Leco); Funded by NSF-EPSCoR RII Track-2 FEC: Insect Cryobiology and Ecophysiology (ICE) Network: integrating genomics, physiology, and modeling

 

MALDI-ToF/ToF-MS (5800^TM, Sciex) housed  in PS 104 (Open Access; please contact Dr. Basile for training) and Funded by NSF-MRI. Used for imaging-MALDI-MS experiment, polymer characterization, glycan detection, peptide mass fingerprint and organic synthesis support.

LC fraction collector

 

MALDI matrix sprayer for imaging MALDI-MS (TM-Sprayer^TM; HTXimaging; NSF-CMI CHE-1611538)

Other supporting instrumentation:

 

---

Gas Chromatograph-(single Quad) MS (GC-MS)  

Electron Ionization and Chemical Ionization, single quadrupole MS with liquid injection autosampler (DSQ, Thermo) (Funds:  NSF CAREER award, 2008)

Microwave radiation heating/microwave flow cell