Basile Lab

Lab and instrumentation

Biodetection and Mass Spectrometry Laboratory

Located in the Physical Sciences Bldg Room 444.

(Mass Spectrometry Instrumentation Lab is located in the Physical Sciences Bldg room 317)

Our Biodetection and Mass Spectrometry Laboratory has adjacent offices for graduate students.  We are equipped with instrumentation and computers to conduct a wide range of analytical MS measurements and data analyses.  Our laboratory also has an in-house microbiology lab (Biosafety Level 2) where we maintain and produce a wide range of bacterial and viral samples for MS biodetection research.

Biodetection and Mass Spectrometry Laboratory


Q-Exactive-HF X a LC-MS/MS high resolution system based on the Orbitrap mass analyzer

Funded by NSF-EPSCoR RII Track-1: Linking microbial life to ecosystem services across Wyoming's dynamic landscape


National Science FoundationWyoming EPSCoR

Orbitrap-MS (Q-Exactive HF-X)  with U-HPLC RP (nano, capillary and micro flows)

nanoESI, heated-ESI (HESI) and APCI sources.

Q-Exactive-HF X a LC-MS/MS high resolution system based on the Orbitrap mass analyzer Funded by NSF-EPSCoR RII Track-1: Linking microbial life to ecosystem services across Wyoming's dynamic landsc

GC-High resolution (ToF) MS  (HRT+; Leco); Funded by NSF-EPSCoR RII Track-2 FEC: Insect Cryobiology and Ecophysiology (ICE) Network: integrating genomics, physiology, and modeling

ht-gcms.jpg

MALDI-ToF/ToF-MS (5800TM, Sciex) housed  in PS 104 (Open Access; please contact Dr. Basile for training) and Funded by NSF-MRI. Used for imaging-MALDI-MS experiment, polymer characterization, glycan detection, peptide mass fingerprint and organic synthesis support.

MALDI 5800

LC fraction collector

nanoLC-offline MALDI spotter

MALDI matrix sprayer for imaging MALDI-MS (TM-SprayerTM; HTXimaging; NSF-CMI CHE-1611538)

NSF-CMI CHE-1611538

Other supporting instrumentation:


Gas Chromatograph-(single Quad) MS (GC-MS)  

GCMS (DSQ; Thermo)

p5210442.jpg

Electron Ionization and Chemical Ionization, single quadrupole MS with liquid injection autosampler (DSQ, Thermo) (Funds:  NSF CAREER award, 2008)

Microwave radiation heating/microwave flow cell

Microwave radiation heating/microwave flow cell

 
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