Contact Us

The Center for Advanced Scientific Instrumentation
Science Initiative Building (SIB) FIrst Floor
Department 4325
Laramie, WY 82071
www.uwyo.edu/science-initiative/casi
E-mail: casi@uwyo.edu


"spINBRE SuperRes": Olympus Spinning Disc Confocal Microscope with Super Resolution (120 nm) 

 

This high-resolution Olympus Spin-SR System boasts an astonishing 120 nm resolution, pushing the boundaries of light microscopy and revealing the intricate details of samples with unparalleled precision and clarity. Moreover, this instrument empowers researchers to observe live specimens in real time, allowing them to delve into the dynamic realm of biological processes. Additionally, modern software enhances data visualization, making it user-friendly and adaptable to individual research protocols. Its exceptional resolution enables scientists to peer deeper into the fundamental elements of life and accelerate UW's life science research. This instrument was purchased with funds provided by NIH Wyoming INBRE that is supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under Grant # 2P20GM103432.  User rates are subsidized by the same award. 

 

spin sr

Instrument Highlights

  • Super Resolution:

The IXplore SpinSR Super Resolution Microscope redefines imaging capabilities, achieving 120 nm XY resolution. This means researchers can observe intricate cellular structures with unmatched clarity well beyond the limits of conventional optical microscopes.

  • Real-Time Imaging for Live Specimens:

The cutting-edge OSR algorithms provide instant super-resolution images, eliminating delays caused by frame averaging or reconstruction. This real-time capability enables live cell experiments and delivers rapid results.

  • Easy Research Integration:

Transition effortlessly between widefield, confocal, and super-resolution imaging with a single click. The IXplore SpinSR system streamlines research by adapting to individuals’ existing experiments and sample protocols. Plus, enhance collected image data with the efficient workflows and advanced analysis tools of CellSens software.

  • Improved Intracellular Clarity:

To prevent out-of-focus fluorescence from blurring the images. This microscope system incorporates a confocal optical system, allowing sharp, super-resolution imaging even in thick samples without the need for special fluorophores or buffers.

  • Enhanced Data Visualization:

Combine TruSight deconvolution with the super resolution for remarkably clear images, eliminating Z-axis blur and providing cleaner three-dimensional insights. High-speed image processing with TruSight deconvolution is seamlessly integrated with Olympus Super Resolution.

  • Efficient Two-Color Imaging:

The IXplore SpinSR system facilitates fast two-color localization super-resolution imaging, using existing fluorophores and laser lines for more efficient experiments.

Specifications

Feature Specification
Resolution
120 nm
Microscope Model Inverted Olympus IX83
Confocal Scan Head Yokogawa W1 Spinning Disc
Spinning Discs 1
50um pinhole disc for conventional confocal imaging
Spinning Discs 2
New SoRa disc for super-resolution live cell imaging (3x increased brightness over conventional 50um disc)
Lasers
405nm, 488nm, 561nm, 640nm
Objective Lenses
40x, 60x, 100x Silicone Oil
Camera
Two sCMOS cameras for simultaneous two channel, high-speed imaging
Imaging Modes
DIC and Brightfield
Illumination Solutions
High speed FRAP/photostimulation system
Live Imagine System
OkoLabs heating/cooling incubation system for live cell imaging from 5C to 60C
Location
Science Initiative Building 1305 Bay 2
Fee
Listed in Rates

 

Contact Info & Location

 

Instrument Manager: Tim Deibert, tdeibert@uwyo.edu 

Instrument Location: Science Initiative Building Bay 2, 1305

Contact Us

The Center for Advanced Scientific Instrumentation
Science Initiative Building (SIB) FIrst Floor
Department 4325
Laramie, WY 82071
www.uwyo.edu/science-initiative/casi
E-mail: casi@uwyo.edu