Basile Lab
Basile Research Group
Franco Basile, Ph.D., Principal Investigator
Dept 3838
Department of Chemistry
Department of Chemistry
University of Wyoming
1000 E. University Ave.
Office (307) 766-4376
Laramie, WY 82071
Email: basile@uwyo.edu
Imaging MALDI-ToF-MS and imaging-MALDESI-Quadrupole-Orbitrap MS:
imaging-MS (UV-MALDESI-Q-Orbitrap-MS)
Whole-body bumblebee imaging-MS (UV-MALDESI-Q-Orbitrap-MS)
On-tissue metabolite derivatization for improved S/N metabolite imaging-MS
TDD: a fast (30 s) non-enzymatic and on-tissue digestion technique compatible with imaging-MS
imaging MALDI-MS of the TDD products of lysozyme: Detected peptides are from site-specific cleavages at Asp and Cys. Images of the spatial distribution of these peptide products are shown below in a proof-of-principle experiment demonstrating applicability of this approach for on-tissue protein id and tissue imaging by MALDI-MS. The solvent-free nature of the TDD process prevents product delocalization, as is the case in the standard protocol using on-tissue trypsin digest. Read our article in Analytical Chem. (2017).
on-tissue TDD of brain tissue and imaging MALDI-MS. Comparison with on-tissue trypsin digestion
Basile Lab
Basile Research Group
Franco Basile, Ph.D., Principal Investigator
Dept 3838
Department of Chemistry
Department of Chemistry
University of Wyoming
1000 E. University Ave.
Office (307) 766-4376
Laramie, WY 82071
Email: basile@uwyo.edu