1000 E. University Avenue
Physical Science Bldg 403
Laramie, WY 82071
The area of research of the Basile Group at the University of Wyoming is Analytical Chemistry with a focus on the application of Mass Spectrometry to analyze a variety of biological and environmental samples. Specifically, our research is aimed at developing rapid biological sample preparation techniques suitable for proteomic measurements that can be implemented in field-portable instrumentation. These techniques are based on the use of non-enzymatic steps to lyse/digest biological samples to a stage that is suitable for MS analysis. Our laboratory pioneered the use of microwave heating for non-enzymatic digestion of proteins at aspartic acid and disulfide bonds and other non-enzymatic site-specific techniques that reduce digestion times from hours to seconds.
Research conducted by the Basile Group also focuses on alternative energy sources like methane production from bacteria residing in coal-bed deposits. Research in this area is geared at performing broad spectrum analysis of soluble matter in coal sludge and proteomic analysis of methanogenic bacteria.