Higher Accuracy Flu Testing Kits

PI: Debashis Dutta

Project Name: Development of a Sensitive Lateral Flow Immunoassay Device for Analyzing Nasal Swab Samples Collected on a Dissolvable Surface 

Graduate Student: Amit Acharzo

Influenza (flu) infections are commonly diagnosed using Rapid Influenza Diagnostic Tests or RIDTs that are inexpensive, do not require specialized equipment, and can provide results within 15 minutes. RIDTs, however, often produce false negative results due to their poor sensitivity requiring that their outcomes be validated with advanced tests. In this project, we demonstrate over 20-fold improvement in the detection limit for flu antigens collected on a dissolvable nasal swab surface and analyzed using our proprietary testing platform. The proposed method also reduces reliance on user skills for performing the tests, cutting down variability in test results.

How this Research Affects the Public

Problem

Current Rapid Influenza Diagnostic Tests (RIDTs) often produce a false negative.

Solution/Product

In this project, we propose to improve RIDT sensitivity by developing a Lateral Flow Immunoassay (LFIA) device capable of analyzing nasal swab samples collected on a dissolvable surface. The use of a dissolvable surface will cut down the liquid volume requirement into which the swab sample is transferred from 350-500 μL to 20-50 μL allowing analysis of the entire specimen rather than a small fraction of it. 

The prototypes for the nasal swab with a dissolvable sampling surface and the proprietary testing platform have been successfully developed. We are currently evaluating the performance of these prototypes using clinical samples in collaboration with UW Student Health Services and Laramie Pediatrics clinic. 

 

Wyoming Impact

Our higher RIDT sensitivity tests kits will eliminate the need for double checking the test results, saving $100-200 in testing cost per incidence and allowing improved disease treatment/management through access to reliable test results within 15 minutes.