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Basile Lab

Basile Research Group

Franco Basile, Ph.D., Principal Investigator

Dept 3838

Department of Chemistry

Department of Chemistry

University of Wyoming

1000 E. University Ave.

Office (307) 766-4376

Laramie, WY 82071

Email: basile@uwyo.edu

Alsike Research Group

Imaging MS

Imaging MALDI-MS and Thermal Decomposition/Digestion (TDD): towards on-tissue digestion and on-tissue protein identification

This research project is funded by the National Science Foundation, NSF-CMI: "Removal of time and fidelity constraints in MALDI-MS imaging of proteins in tissue"

NSF

Project aims at developing a fast (30 s) non-enzymatic and on-tissue digestion technique compatible with imaging-MS

 TDD imaging MALDI-MS

imaging MALDI-MS of the TDD products of lysozyme: Detected peptides are from site-specific cleavages at Asp and Cys.  Images of the spatial distribution of these peptide products are shown below in a proof-of-principle experiment demonstrating applicability of this approach for on-tissue protein id and tissue imaging by MALDI-MS. The solvent-free nature of the TDD process prevents product delocalization, as is the case in the standard protocol using on-tissue trypsin digest. Read our article in Analytical Chem. (2017).

 

Proof-of-concept on-tissue TDD of brain tissue and imaging MALDI-MS. Comparison with on-tissue trypsin digestion

tdd_digest_brain_ims.png

trypsin_digest_brain_ims.png

Contact Us

Basile Lab

Basile Research Group

Franco Basile, Ph.D., Principal Investigator

Dept 3838

Department of Chemistry

Department of Chemistry

University of Wyoming

1000 E. University Ave.

Office (307) 766-4376

Laramie, WY 82071

Email: basile@uwyo.edu

Alsike Research Group

1000 E. University Ave. Laramie, WY 82071
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