Imaging MS

Imaging MALDI-ToF-MS and imaging-MALDESI-Quadrupole-Orbitrap MS:

• Thermal Decomposition/Digestion (TDD): rapid on-tissue digestion for on-tissue protein identification
• Whole body bee metabolite imaging-MS
• On-tissue metabolite derivatization to enhance signal S/N
  
• Root exudate imaging-MS

Whole body bumble bee imaging-MS (UV-MALDESI-Q-Orbitrap-MS). From top to bottom: head, thorax, and abdomen.

On-tissue metabolite derivatization for improved S/N metabolite imaging-MS

 

 

 

TDD: a fast (30 s) non-enzymatic and on-tissue digestion technique compatible with imaging-MS

 

imaging MALDI-MS of the TDD products of lysozyme: Detected peptides are from site-specific cleavages at Asp and Cys.  Images of the spatial distribution of these peptide products are shown below in a proof-of-principle experiment demonstrating applicability of this approach for on-tissue protein id and tissue imaging by MALDI-MS. The solvent-free nature of the TDD process prevents product delocalization, as is the case in the standard protocol using on-tissue trypsin digest. Read our article in Analytical Chem. (2017).

 

on-tissue TDD of brain tissue and imaging MALDI-MS. Comparison with on-tissue trypsin digestion