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Stable Isotope Facility

University of Wyoming

 

Elemental Analyzer (EA-IRMS)

δ13C and δ15N of Organic and Inorganic Material

Carbon and nitrogen containing compounds occur in both organic (such as plants and animals) and inorganic (such as soils) systems.  δ13 and δ15N ratios of the material can be determined by isotopic analysis of the CO2 and N2 generated by the combustion of the material. At SIF, the homogenized sample is accurately weighed into a tin capsule and loaded into the autosampler attached to an elemental analyzer.  The EA is coupled to an IRMS through an open split interface.  When a sample is dropped into the EA, the EA automatically processes the sample, combusting the sample to generate product gases that are then separated using the gas chromatograph.  The separated gases (CO2 and N2) are then pass through the open split interface to the IRMS for isotopic analysis (EA-IRMS).

Typical sample size:    1-2 mg (plant), 0.5-1 mg (animal), 5-20 mg (soil)


Elemental Analyzer Scheme


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Stable Isotope Facility

Berry Center RM 214

Laramie, WY 82071

Phone: (307) 766-6373

Email: uwyosif@uwyo.edu

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1000 E. University Ave. Laramie, WY 82071
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