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Stable Isotope Facility

University of Wyoming

Combustion (GC-C-IRMS) Pyrolysis (GC-TC-IRMS)

 

Compound Specific Isotope Analysis

Compound specific isotope analysis (CSIA) analyzes specific compounds for δ13C, δ15N, δ18O, and δ2H ratios through the use of gas chromatograph separation coupled to either combustion or pyrolysis interfaces.  δ18O and δ2H ratios of a sample are determined by the analysis of the CO and H2 generated when the sample flows through the pyrolysis reactor.  For δ13C and δ15N analysis, the CO2 and N2 are generated by the combustion of the sample. At SIF, the compound specific analysis technique used to generate CO and H2 for δ18O and δ2H ratios is as follows: after a sample mixture is injected into a gas chromatograph, the mixture is separated through the gas chromatograph column, the individual compounds pass through a pyrolysis reactor before being sent to the IRMS for analysis.  For compound specific analysis of samples for δ13C or δ15N, the same steps apply except the samples are combusted after gas chromatograph separation (GC-C-IRMS).

Typical sample size:    varies

The GC-C-IRMS used for compound specific analysis is available to qualified University of Wyoming researchers.  UWSIF DOES NOT perform these analyses. 


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Stable Isotope Facility

Berry Center RM 214

Laramie, WY 82071

Phone: (307) 766-6373

Email: uwyosif@uwyo.edu

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1000 E. University Ave. Laramie, WY 82071
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