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Stable Isotope Facility

University of Wyoming

Solid Samples for δ13C / δ15N / δ18O / δ2H analysis

Samples should be thoroughly dried, ground and well mixed. Samples pretreated with acids need to be given at least 5-8 rinses with distilled water to get rid of all the acid in the sample.  We can load your natural abundance samples  ($1/sample). If you have enriched samples or decide to load your natural abundance samples yourselves please use 96 well plastic trays. Samples should be loaded according to the tray loading template. Please leave the well number marked for lab standards empty.

If submitting soil samples please contact SIF before sending. 

If submitting samples for solid hydrogen analysis please contact SIF before sending.  Hydrogen analysis can be complicated because hydrogen readily exchanges with atmospheric hydrogen.  Special procedures must be followed in order to get accurate results.  Special preparation charges may apply.

Load samples in the proper size and type of capsules. You can order the 3.5x5 mm, 4x6 mm, and 5x9 mm capsules from Costech.

    *Use the smallest capsule size that will hold the sample with no leakage.

    * Use tin capsules for δ13C / δ15N analysis

    * Use silver capsules for δ18O / δ2H analysis

    * Make sure the capsule does not have any protrusions.

Keep the working area, weighing table and spatula and forceps very clean. Wipe the forceps and spatulas with Kimwipe in-between subsequent samples. Capsules should be folded and crushed into a cube or ball without any protrusions.  Samples that are flat or have protrusions tend to get stuck in the sample carousel. Record the final weight after the capsule is sealed. For elemental C and N analysis, the same sample loading procedure should be followed.  Also, please ensure that no sample is leaking from the tin.

Required sample size:

    * C/N please refer to our Excel sample calculator.

    * O/H - 50 to 400 ug in sample

To prevent samples mixing in the plate during shipping cover the loaded samples with the cardboard or card stock, cut to fit, and place the lid on top.  Tape the lid down.  Pack in box with packing material.


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